Transposition and target preferences of an active nonautonomous DNA transposon nDart1 and its relatives belonging to the hAT superfamily in rice
Transposition and target preferences of an active nonautonomous DNA transposon nDart1 and its relatives belonging to the hAT superfamily in rice
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DOI：
10.1007/s00438-010-0569-9
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发表时间：
2010-11-01
影响因子：
3.1
通讯作者：
Iida, Shigeru
中科院分区：
文献类型：
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作者：
Takagi, Kyoko;Maekawa, Masahiko;Iida, Shigeru
hAT超家族中的非自主性nDart 1元件是水稻中少数几个活跃的DNA转座子之一。它的转座可以通过与含有活性自主元件aDart 1的线交叉来诱导，并通过分离aDart 1来稳定。在nDart 1促进的基因标记中不应发生体细胞克隆变异，因为没有组织培养参与nDart 1激活。通过转座子展示分析，我们研究了nDart 1相关元件在含有aDart 1的突变的villopyl-v植物的自交后代中的活性。尽管水稻基因组中存在多种与nDart 1相关的元件，但只有nDart 1 -3亚组元件，特别是nDart 1 -0和nDart 1 -3，被发现频繁转座并整合到几乎整个基因组的各个位点上，而且一小部分转座元件被发现传递到下一代。超过一半的新整合元素被确定为nDart 1 -0。对新插入位点的分析显示，nDart 1 -3亚组元件主要整合到单拷贝区域中。超过60%的转座元件被插入到包含推定编码区及其0.5-kb侧翼片段的基因区域中，并且它们中的大约三分之二在推定起始密码子前面的0.5-kb区域内，即，启动子近端基因区。nDart 1 -3亚组元件的这些特征可能适合于建立一个有效的、无体细胞克隆变异的水稻功能基因组学基因标签系统。
The nonautonomous nDart1 element in the hAT superfamily is one of a few active DNA transposons in rice. Its transposition can be induced by crossing with a line containing an active autonomous element, aDart1, and stabilized by segregating aDart1. No somaclonal variation should occur in nDart1-promoted gene tagging because no tissue culture is involved in nDart1 activation. By transposon display analysis, we examined the activities of nDart1-related elements in the selfed progeny of a mutable virescent pyl-v plant containing aDart1. Although various nDart1-related elements are present in the rice genome, only nDart1-3 subgroup elements, nDart1-0 and nDart1-3 in particular, were found to be transposed frequently and integrated into various sites almost all over the genome, and a fraction of the transposed elements were found to be transmitted to the next generation. More than half of the newly integrated elements were identified as nDart1-0. Analysis of the newly inserted sites revealed that the nDart1-3 subgroup elements were predominantly integrated into single-copy regions. More than 60% of the transposed elements were inserted into the genic regions that comprise putative coding regions and their 0.5-kb flanking segments, and approximately two-thirds of them were within the 0.5-kb area in front of the putative initiation codons, i.e., promoter-proximal genic regions. These characteristic features of nDart1-3 subgroup elements seem to be suitable for developing an efficient and somaclonal variation-free gene tagging system for rice functional genomics.