Effect of Cocaine on Ion Channels and Glutamatergic EPSCs in Noradrenergic Locus Coeruleus Neurons
Effect of Cocaine on Ion Channels and Glutamatergic EPSCs in Noradrenergic Locus Coeruleus Neurons
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可卡因对去甲肾上腺素能蓝斑神经元离子通道和谷氨酸能 EPSC 的影响
DOI：
10.1007/s12031-013-0159-5
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发表时间：
2014-07-01
影响因子：
3.1
通讯作者：
Xu, Z. Q. D.
中科院分区：
文献类型：
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作者：
Liu, L. N.;Zhu, F. P.;Xu, Z. Q. D.
蓝斑区是参与可卡因成瘾的重要脑干区域。然而，阐明可卡因如何调节LC神经元活动的证据仍然不完整。在这里，我们对大脑切片进行了完整的记录，以评估可卡因对蓝斑神经元中钠（Na+）、钾（K+）、钙（Ca2+）通道和谷氨酸能突触传递的影响。局部使用可卡因可显著且可逆地降低自发性放电率，但对动作电位振幅、上升时间、衰减时间和去肾上腺素能蓝斑神经元半宽无影响。此外，可卡因减弱了钠电流，但对钾和钙电流没有影响。n -甲基-d-天冬氨酸受体介导的兴奋性突触后电流被神经肽丙氨酸而非可卡因所减少。这些数据表明，可卡因对蓝斑神经元的自发活动和钠电流有抑制作用。因此，蓝斑神经元钠通道的神经调节可能在药物成瘾中起重要作用。
The locus coeruleus (LC) is an important brainstem area involved in cocaine addiction. However, evidence to elucidate how cocaine modulates the activity of LC neurons remains incomplete. Here, we performed whole recordings in brain slices to evaluate the effects of cocaine on the sodium (Na+), potassium (K+), calcium (Ca2+) channels, and glutamatergic synaptic transmission in the locus coeruleus neurons. Local application of cocaine significantly and reversibly reduced the spontaneous firing rate but did not affect action potential amplitude, rising time, decay time, or half width of noradrenergic locus coeruleus neurons. Moreover, cocaine attenuated the sodium current but did not affect potassium and calcium currents. The N-methyl-d-aspartate receptor mediated excitatory postsynaptic currents were reduced by neuropeptide galanin but not cocaine. All those data demonstrate that cocaine has inhibitory effect on the spontaneous activities and sodium current in locus coeruleus neurons. Therefore, neuromodulation of sodium channel in locus coeruleus neurons may play an important role in drug addiction.