SELECTIVE INTERACTIONS OF ELECTROLECTINS FROM EEL ELECTRIC ORGAN AND MOUSE THYMUS WITH MOUSE IMMATURE THYMOCYTES
SELECTIVE INTERACTIONS OF ELECTROLECTINS FROM EEL ELECTRIC ORGAN AND MOUSE THYMUS WITH MOUSE IMMATURE THYMOCYTES
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DOI：
10.1016/0165-2478(83)90052-4
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发表时间：
1983-01-01
影响因子：
4.4
通讯作者：
TEICHBERG, VI
中科院分区：
文献类型：
--
作者：
LEVI, G;TEICHBERG, VI
纯电凝集素a。来自电鳗电器官的- d -半乳糖苷结合凝集素选择性地凝集在小鼠胸腺细胞亚群中。在胎牛血清上沉淀1g可将该细胞群与非凝集细胞分离。根据.theta的密度鉴定凝集细胞为未成熟胸腺细胞。它们表面携带的抗原，它们的有丝分裂活性以及对植物血凝素有丝分裂作用的反应缺失。未成熟的小鼠胸腺细胞结合内源性小鼠胸腺凝集素（MTL），一种与鳗鱼电凝集素具有相同糖类特异性的蛋白质，并与之发生免疫交叉反应。MTL由小鼠胸腺网织细胞在组织培养中分泌，在胸腺细胞耗尽后其比活性显著增加。提示MTL可能定位于胸腺上皮。本文结合近年来有关鳗鱼电凝集素对实验性自身免疫性重症肌无力具有预防和治疗作用的研究结果，对上述结果进行了讨论。
The pure electrolectin, a .beta.-D-galactoside binding lectin from the electric organ of the electric eel E. electricus agglutinated selectively a subpopulation of mouse thymocytes. This cell population could be separated from non-agglutinated cells by 1 g sedimentation over fetal calf serum. The agglutinated cells were identified as immature thymocytes on the basis of the density of .theta.-antigen they bear on their surfaces, their mitotic activity and the absence of response to the mitogenic action of phytohemagglutinin. The immature mouse thymocytes bound the endogenous mouse thymic lectin (MTL) a protein that displays the same saccharide specificity as the eel electrolectin and with which it cross-reacts immunologically. MTL is secreted by mouse thymic reticulocytes in tissue culture and its specific activity is markedly increased after depleting the thymus of its thymocytes. The possible localization of MTL in the thymic epithelium was indicated. These results are discussed in the light of recent findings that the eel electrolectin has prophylactic and therapeutic actions on experimental autoimmune myasthenia gravis in rabbits.