Adipophilin expression in cutaneous malignant melanoma
Adipophilin expression in cutaneous malignant melanoma
复制标题
DOI：
10.1111/cup.12868
复制
发表时间：
2017-03-01
影响因子：
1.7
通讯作者：
Murata, Shin-ichi
中科院分区：
文献类型：
--
作者：
Fujimoto, Masakazu;Matsuzaki, Ibu;Murata, Shin-ichi
背景：脂肪生成途径在包括黑色素瘤在内的癌细胞中上调。然而，细胞脂质在黑色素细胞病变中的病理意义尚未确定。在这项研究中，我们通过免疫组织化学分析包裹在脂滴上的亲脂素（ADP）来评估黑素细胞痣（MNs）和恶性黑素瘤的胞浆内脂滴。方法：对100例原发性皮肤黑色素细胞病变[33例MNs， 17例原位黑色素瘤（MIS）， 50例浸润性黑色素瘤（IMs）]进行ADP免疫染色。半定量记录各病例ADP染色强度评分（IS）和比例评分（PS），评分范围为0 ~ 3+。结果：27例原发性皮肤黑色素细胞病变中ADP高表达（IS2/3+和PS2/3+），包括23例IMs、3例MISs和1例MN。因此，高ADP表达与恶性肿瘤相关（38.8% vs. 3.0%; p < 0.0001）。在IMs中，pT3/4期ADP高表达高于pT1/2期（63.3%比23.8%,p = 0.01）， 3/4期高于1/2期（76.9%比36.8%,p = 0.02）。结论：在我们的队列中，大多数高ADP表达的黑色素细胞病变为恶性黑色素瘤。因此，ADP表达可作为恶性黑色素瘤的敏感诊断标志物。
Background: The lipogenic pathway is upregulated in cancer cells, including melanomas. However, the pathological significance of cellular lipids in melanocytic lesions has yet to be determined. In this study, we evaluated intracytoplasmic lipid droplets in melanocytic nevi (MNs) and malignant melanomas via immunohistochemical analysis of adipophilin (ADP), which coats lipid droplets.Methods: One hundred primary cutaneous melanocytic lesions [33 MNs, 17 melanomas in situ (MIS), and 50 invasive melanomas (IMs)] were immunostained for ADP. The intensity score (IS) and proportion score (PS) of ADP staining in each case was recorded semiquantitatively on a scale of 0 to 3+.Results: High ADP expression (IS2/3+ and PS2/3+) was observed in 27 primary cutaneous melanocytic lesions that consisted of 23 IMs, three MISs, and one MN. Consequently, high ADP expression was associated with malignancy (38.8% vs. 3.0%; p < 0.0001). Among the IMs, high ADP expression was more prevalent in pT3/4 than pT1/2 (63.3% vs. 23.8%; p = 0.01) and Stage 3/4 than Stage 1/2 (76.9% vs. 36.8%; p = 0.02).Conclusions: The majority of the melanocytic lesions with high ADP expression were malignant melanomas in our cohort. Therefore, ADP expression may serve as a sensitive diagnostic marker for malignant melanoma.