STUDIES ON VINBLASTINE-INDUCED AUTOPHAGOCYTOSIS IN MOUSE-LIVER .2. ORIGIN OF MEMBRANES AND ACQUISITION OF ACID-PHOSPHATASE
STUDIES ON VINBLASTINE-INDUCED AUTOPHAGOCYTOSIS IN MOUSE-LIVER .2. ORIGIN OF MEMBRANES AND ACQUISITION OF ACID-PHOSPHATASE
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DOI：
10.1007/bf00493232
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
REUNANEN, H
中科院分区：
文献类型：
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作者：
HIRSIMAKI, P;REUNANEN, H
用长春碱（VBL，50 mg/kg，i. p.）在小鼠肝细胞中。使用未缓冲的OsO 4，非常强烈的染色观察到在高尔基体的外池，也经常在明显注定要形成AV的双膜之间的空腔和新形成的AV的双膜之间的空腔。在限制AV的膜中可能发生类似于在高尔基池观察到的转化过程。AV外膜的转化独立于与溶酶体的融合而发生。肌苷二磷酸酶活性定位在池和内质网膜，偶尔在高尔基体的最里面的池。结合未缓冲OsO_4染色的结果表明，大多数AV的膜来源于不具有肌苷二磷酸酶活性的内质网的转化的光滑表面池。酸性磷酸酶活性定位于溶酶体，偶尔在高尔基体的最里面的池，一些新形成的AV和大多数老的单膜限制AV的双膜之间。VBL不能阻止溶酶体与AV的融合。
The origin of the membranes of autophagic vacuoles (AV) and acquisition of acid phosphatase into AV were studied in vinblastine-induced autophagocytosis (VBL, 50 mg/kg, i.p.) in mouse hepatocytes. Using unbuffered OsO4, very intense staining was observed in the outer cisternae of the Golgi apparatus and also frequently in the cavity between the double membranes obviously destined to form AV and in the cavity between the double membranes of newly formed AV. There may occur a transformation process in the membranes limiting an AV analogous to that observed at the Golgi cisternae. The transformation of the outer AV membrane occurs independently of fusion with lysosomes. Inosine diphosphatase activity was localized within the cisternae and on the membranes of the endoplasmic reticulum and occasionally within the innermost cisterna of the Golgi apparatus. The results together with the unbuffered OsO4-staining pattern suggest that the membranes of most AV are derived from the transformed smooth surfaced cisternae of the endoplasmic reticulum which do not have inosine diphosphatase activity. Acid phosphatase activity was localized in lysosomes, occasionally within the innermost cisternae of the Golgi apparatus, between the double membranes of a few newly formed AV and within most older single membrane-limited AV. VBL did not prevent the fusion of lysosomes with AV.