Distinct redox regulation in sub-cellular compartments in response to various stress conditions in Saccharomyces cerevisiae.
Distinct redox regulation in sub-cellular compartments in response to various stress conditions in Saccharomyces cerevisiae.
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DOI：
10.1371/journal.pone.0065240
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Dawes IW
中科院分区：
文献类型：
--
作者：
Ayer A;Sanwald J;Pillay BA;Meyer AJ;Perrone GG;Dawes IW
对许多生长和应激条件的反应被认为是通过改变细胞的氧化还原状态而起作用的。然而，在生长和应力过程中的pH调节的氧化还原状态的直接测量从未进行过。用荧光探针roGFP 2和pHluorin测定了酿酒酵母细胞器的氧化还原状态（E GSH）。特别是，我们研究了细胞器的氧化还原状态的变化，在各种生长和应激条件下，以更好地了解氧化还原，氧化和环境应激反应系统之间的关系。在各种培养基中，测定了细胞质、线粒体基质和过氧化物酶体在指数期和稳定期的E GSH值。这些值（−340至−350 mV）比以前报告的要低。有趣的是，亚细胞氧化还原状态保持不变，当细胞受到挑战与以前报道的影响氧化还原稳态的压力。只有过氧化氢和热应激显着改变细胞器的氧化还原状态。过氧化氢应激改变了谷胱甘肽二硫化物/谷胱甘肽对（GSSG，2 H +/2GSH）和pH值的氧化还原状态。从中度过氧化氢应激的恢复是最迅速的细胞质中，其次是线粒体基质，与过氧化物酶体最不能够恢复。相反，在热强制降解过程中观察到的大部分氧化还原位移是由pH值的变化引起的，而不是GSSG，2 H +/2GSH对。这项研究提出了第一个直接测量的pH值调节氧化还原状态的亚细胞室在生长和应力条件下。氧化还原状态在细胞器中受到明显的调节，提出的数据挑战了氧化还原状态的扰动是许多应激条件下反应的核心的概念。
Responses to many growth and stress conditions are assumed to act via changes to the cellular redox status. However, direct measurement of pH-adjusted redox state during growth and stress has never been carried out. Organellar redox state (E GSH) was measured using the fluorescent probes roGFP2 and pHluorin in Saccharomyces cerevisiae. In particular, we investigated changes in organellar redox state in response to various growth and stress conditions to better understand the relationship between redox-, oxidative- and environmental stress response systems. E GSH values of the cytosol, mitochondrial matrix and peroxisome were determined in exponential and stationary phase in various media. These values (−340 to −350 mV) were more reducing than previously reported. Interestingly, sub-cellular redox state remained unchanged when cells were challenged with stresses previously reported to affect redox homeostasis. Only hydrogen peroxide and heat stress significantly altered organellar redox state. Hydrogen peroxide stress altered the redox state of the glutathione disulfide/glutathione couple (GSSG, 2H+/2GSH) and pH. Recovery from moderate hydrogen peroxide stress was most rapid in the cytosol, followed by the mitochondrial matrix, with the peroxisome the least able to recover. Conversely, the bulk of the redox shift observed during heat stress resulted from alterations in pH and not the GSSG, 2H+/2GSH couple. This study presents the first direct measurement of pH-adjusted redox state in sub-cellular compartments during growth and stress conditions. Redox state is distinctly regulated in organelles and data presented challenge the notion that perturbation of redox state is central in the response to many stress conditions.
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影响因子：
56.9
作者：
HWANG, C;SINSKEY, AJ;LODISH, HF
通讯作者：
LODISH, HF
线粒体膜间隙中的氧化还原环境与细胞质和基质是分开的
影响因子：
4.8
作者：
Hu, Jingjing;Dong, Lixue;Outten, Caryn E.
通讯作者：
Outten, Caryn E.
DOI：
10.1073/pnas.93.10.5116
发表时间：
1996-05-14
影响因子：
11.1
作者：
Davidson, JF;Whyte, B;Schiestl, RH
通讯作者：
Schiestl, RH
影响因子：
48
作者：
Gutscher, Marcus;Pauleau, Anne-Laure;Dick, Tobias P.
通讯作者：
Dick, Tobias P.
影响因子：
4.1
作者：
Garcera, Ana;Barreto, Lina;Herrero, Enrique
通讯作者：
Herrero, Enrique