Fetal cells in the murine maternal lung have well-defined characteristics and are preferentially located in alveolar septum.
Fetal cells in the murine maternal lung have well-defined characteristics and are preferentially located in alveolar septum.
复制标题
小鼠母肺中的胎儿细胞具有明确的特征，并且优先位于肺泡间隔中。
DOI：
10.1089/scd.2010.0518
复制
发表时间：
2012
影响因子：
4
通讯作者：
Bianchi,DianaW
中科院分区：
文献类型：
--
作者：
Johnson,KirbyL;Stroh,Helene;Tadesse,Serkalem;Norwitz,ErrolR;Richey,Lauren;Kallenbach,LisaR;Bianchi,DianaW
胚胎细胞向母体器官的转移发生在小鼠和人类妊娠中。聚合酶链反应和流式细胞术等技术不允许研究胎儿细胞形态或解剖位置。使用一个绿色荧光蛋白（GFP）转基因小鼠模型，我们的目标是确定是否GFP+信号来自完整或降解的胎儿细胞，以及它们是否有一个特征性的外观和位置在母体肺。将四只野生型雌性小鼠与GFP转基因纯合子雄性小鼠交配，并在e16-18天进行研究。对照组为与野生型雄性交配的2只雌性动物。记录母体肺内每个GFP+物体的形态外观和解剖位置。GFP信号足够明亮，在没有抗GFP抗体的情况下可以可视化，并通过共聚焦显微镜证实与荧光伪影分离。在检测到的438个GFP+对象中，375个（85.6%）来自完整细胞，63个（14.4%）来自非细胞。观察到四种不同类型的完整细胞。其中，23.2%具有单核形态，具有相对较大的细胞核和GFP+细胞质（A组）。另一组细胞（10.1%）具有单核细胞形态和足细胞延伸（B组）。其余细胞具有破碎的细胞核或细胞质。完整细胞和非细胞碎片主要定位于母体肺泡隔（P<0.0001）。这项研究表明，胎儿GFP+细胞主要位于肺泡隔，并具有特征性的形态，但目前还不清楚这些是否代表不同类别的细胞或降解细胞。然而，在研究肺生物学和修复时，应考虑母体肺中自然获得的胎儿细胞群。
The transfer of fetal cells to maternal organs occurs in mouse and human pregnancy. Techniques such as polymerase chain reaction and flow cytometry do not permit study of fetal cell morphology or anatomic location. Using a green fluorescent protein (GFP) transgenic mouse model, our objective was to determine whether GFP+ signal emanates from intact or degraded fetal cells, and whether they have a characteristic appearance and location within maternal lung. Four wild-type female mice were mated to males homozygous for theGfptransgene and studied at days e16–18. Controls were 2 females mated to wild-type males. Morphologic appearance and anatomic position of each GFP+ object within maternal lung was recorded. GFP signals were sufficiently bright to be visualized without anti-GFP antibody and were confirmed by confocal microscopy to be separate from fluorescent artifact. Of 438 GFP+ objects detected, 375 (85.6%) were from intact cells, and 63 (14.4%) were acellular. Four distinct categories of intact cells were observed. Of these, 23.2% had mononuclear morphology with a relatively large nucleus and GFP+ cytoplasm (Group A). An additional group of cells (10.1%) had mononuclear morphology and podocyte extensions (Group B). The remainder of cells had fragmented nuclei or cytoplasm. Both intact cells and acellular fragments were predominantly localized to the maternal alveolar septum (P<0.0001). This study demonstrates that fetal GFP+ cells are predominantly located in the alveolar septum and have characteristic morphologies, although it remains unclear whether these represent distinct categories of cells or degrading cells. Nevertheless, this naturally acquired population of fetal cells in maternal lung should be considered in studies of lung biology and repair.