Transcriptome profiles reveal that gibberellin-related genes regulate weeping traits in crape myrtle
Transcriptome profiles reveal that gibberellin-related genes regulate weeping traits in crape myrtle
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观赏植物Asparagus setaceus的染色体水平基因组组装、注释和进化分析
DOI：
10.1038/s41438-020-0271-y
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发表时间：
2020-04-01
影响因子：
8.7
通讯作者：
Zhang, Qixiang
中科院分区：
文献类型：
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作者：
Li, Suzhen;Zheng, Tangchun;Zhang, Qixiang
植物结构包括影响和有利于作物的重要性状，以及经济上重要的树木。不同的植物结构提供了自然美。垂垂的观赏植物给人以美感。紫薇抽泣性状的调控机制尚不清楚。为探讨垂垂紫薇和直立紫薇的垂垂性状机制，以表型为基础，对垂垂紫薇和直立紫薇不同器官的转录谱进行了分析。表型和组织学分析表明，垂生植物内胚层细胞缺失，GA3处理可恢复新梢表型。差异表达基因的转录分析和共表达网络分析（WGCNA）表明，GA合成和信号转导途径在哭泣性状中起作用。当GA信号的负向元件LfiGRAS1的表达水平通过病毒诱导的基因沉默（VIGS）降低时，受感染植物的新枝以负向地性方式生长。综合分析表明，赤霉素与其他因子相互作用，对垂紫薇有较强的影响。本研究有助于阐明抽泣性状的调控机制，提高紫薇的育种效率。
Plant architecture includes vital traits that influence and benefit crops, and economically important trees. Different plant architectures provide natural beauty. Weeping ornamental plants are aesthetically appealing to people. The regulatory mechanism controlling the weeping trait is poorly understood in crape myrtle. To investigate the weeping trait mechanism, transcriptional profiling of different organs in weeping and upright crape myrtle was performed based on phenotype. Phenotypic and histological analyses demonstrated that endodermal cells were absent, and that new shoot phenotypes could be rescued by the GA3 treatment of weeping plants. The transcriptional analysis and coexpression network analysis (WGCNA) of differentially expressed genes indicated that GA synthesis and signal transduction pathways play a role in weeping traits. When the expression level of a negative element of GA signaling, LfiGRAS1, was reduced by virus-induced gene silencing (VIGS), new branches grew in infected plants in a negatively geotropic manner. An integrated analysis implied that GA had a strong influence on weeping crape myrtle by interacting with other factors. This study helps to elucidate the mechanism governing the weeping trait and can improve the efficiency of breeding in Lagerstroemia.