Histological validation of myocardial microstructure obtained from diffusion tensor magnetic resonance imaging
Histological validation of myocardial microstructure obtained from diffusion tensor magnetic resonance imaging
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DOI：
10.1152/ajpheart.1998.275.6.h2308
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发表时间：
1998-12-01
影响因子：
4.8
通讯作者：
Forder, J
中科院分区：
文献类型：
--
作者：
Scollan, DF;Holmes, A;Forder, J
扩散张量磁共振成像（MRI）是一种可能的新手段，阐明解剖结构的心肌。与传统的组织学方法相比，它具有几个优点，包括能够快速测量离体、灌注、停搏心脏中的纤维组织，从而避免固定和切片伪影。然而，这种MRI方法的定量验证一直缺乏。在这里，纤维取向估计在相同的位置在同一心脏使用扩散张量MRI和组织学进行比较，在总共两个灌注兔心脏。两种方法的纤维方向在统计学上相似，在任何单一位置平均相差12度。这类似于组织学获得的纤维取向的10度不确定性。此外，在总共7个心脏中进行的成像研究支持肌纤维之外的组织水平，最近描述的心室肌的层状组织。
Diffusion tensor magnetic resonance imaging (MRI) is a possible new means of elucidating the anatomic structure of the myocardium. It enjoys several advantages over traditional histological approaches, including the ability to rapidly measure fiber organization in isolated, perfused, arrested hearts, thereby avoiding fixation and sectioning of artifacts. However, quantitative validation of this MRI method has been lacking. Here, fiber orientations estimated in the same locations in the same heart using both diffusion tensor MRI and histology are compared in a total of two perfused rabbit hearts. Fiber orientations were statistically similar for both methods and differed on average by 12 degrees at any single location. This is similar to the 10 degrees uncertainty in fiber orientation achieved with histology. In addition, imaging studies performed in a total of seven hearts support a level of organization beyond the myofiber, the recently described laminar organization of the ventricular myocardium.