A microfluidic chip integrated with a high-density PDMS-based microfiltration membrane for rapid isolation and detection of circulating tumor cells
A microfluidic chip integrated with a high-density PDMS-based microfiltration membrane for rapid isolation and detection of circulating tumor cells
复制标题
集成高密度 PDMS 微滤膜的微流控芯片，用于快速分离和检测循环肿瘤细胞
DOI：
10.1016/j.bios.2015.04.080
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发表时间：
2015-09-15
影响因子：
12.6
通讯作者：
Zhao, Jianlong
中科院分区：
文献类型：
--
作者：
Fan, Xiaoyun;Jia, Chunping;Zhao, Jianlong
通过尺寸排阻分离循环肿瘤细胞（CTC）是一种广泛研究的技术，其提供了捕获肿瘤细胞而不依赖于细胞表面表达标志物的优点。在这项工作中，我们报告了一种新型的聚二甲基硅氧烷（PDMS）膜过滤器为基础的微型装置的快速和高效的分离CFCs从外周血的发展。通过牺牲转移膜与三明治成型相结合的方法，制备了一种精密的、高孔隙率的PDMS微孔过滤器，并将其集成到微滤芯片中。当从加标血液样品中分离肺癌细胞时，我们实现了> 90%的回收率，具有10 mL/h的相对较高的处理通量。与现有的CTC过滤系统相比，这些系统依赖于低孔隙率的径迹蚀刻过滤器或昂贵的基于光刻的过滤器，我们的微滤芯片不需要复杂的电子束光刻或反应离子蚀刻工艺，因此它提供了一种低成本的替代工具，用于高效的CTC富集和原位分析。因此，这种新的微型装置具有在临床环境中用于常规监测癌症发展和癌症治疗的潜力。(C)2015 Elsevier B. V.版权所有。
Isolation of circulating tumor cells (CTCs) by size exclusion is a widely researched technique that offers the advantage of capturing tumor cells without reliance on cell surface expression markers. In this work, we report the development of a novel polydimethylsiloxane (PDMS) membrane filter-based microdevice for rapid and highly efficient isolation of CFCs from peripheral blood. A precise and highly porous PDMS microfilter was fabricated and integrated into the microfiltration chip by combining a sacrificial transferring film with a sandwich molding method. We achieved > 90% recovery when isolating lung cancer cells from spiked blood samples, with a relatively high processing throughput of 10 mL/h. In contrast to existing CTC filtration systems, which rely on low-porosity track-etch filters or expensive lithography-based filters, our microfiltration chip does not require complex e-beam lithography or the reactive ion etching process, therefore it offers a low-cost alternative tool for highly efficient CTC enrichment and in situ analysis. Thus, this new microdevice has the potential for use in routine monitoring of cancer development and cancer therapy in a clinical setting. (C) 2015 Elsevier B.V. All rights reserved.