Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human cardiac physiology and pathophysiology
Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human cardiac physiology and pathophysiology
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DOI：
10.1038/nprot.2008.229
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发表时间：
2009-01-01
期刊：
影响因子：
14.8
通讯作者：
Goumans, Marie-Jose
中科院分区：
文献类型：
--
作者：
Smits, Anke M.;van Vliet, Patrick;Goumans, Marie-Jose
迄今为止，还没有合适的体外模型来研究人类成年心脏细胞生物学。尽管胚胎干细胞能够在体外分化为心肌细胞，但这一过程的效率非常低。其他将祖细胞分化为搏动心肌细胞的方法依赖于与大鼠新生心肌细胞共培养，这使得研究人类心肌细胞分化和（病理）生理学变得困难。在这里，我们开发了一种从心脏手术废物或胎儿心脏组织中有效分离和扩增人类心肌祖细胞（CMPC）的方法。此外，我们提供了详细的体外方案，用于将 CMPC 有效分化为心肌细胞，效率很高（分化的 80-90%）。一旦 CMPC 快速分裂（类似于分离后 1 个月），3-4 周内即可实现分化。
To date, there is no suitable in vitro model to study human adult cardiac cell biology. Although embryonic stem cells are able to differentiate into cardiomyocytes in vitro, the efficiency of this process is very low. Other methods to differentiate progenitor cells into beating cardiomyocytes rely on coculturing with rat neonatal cardiomyocytes, making it difficult to study human cardiomyocyte differentiation and (patho) physiology. Here we have developed a method for efficient isolation and expansion of human cardiomyocyte progenitor cells (CMPCs) from cardiac surgical waste or alternatively from fetal heart tissue. Furthermore, we provide a detailed in vitro protocol for efficient differentiation of CMPCs into cardiomyocytes with great efficiency (80-90% of differentiation). Once CMPCs are rapidly dividing (similar to 1 month after isolation), differentiation can be achieved in 3-4 weeks.