Bulleidia extructa gen. nov., sp nov., isolated from the oval cavity
Bulleidia extructa gen. nov., sp nov., isolated from the oval cavity
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DOI：
10.1099/00207713-50-3-979
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发表时间：
2000-05-01
影响因子：
2.8
通讯作者：
Wade, WG
中科院分区：
文献类型：
--
作者：
Downes, J;Olsvik, B;Wade, WG
从晚期牙周炎中分离出5株无孢子厌氧革兰氏阳性杆菌（4株）和1株牙槽脓肿菌)，对其进行表型和遗传鉴定。通过16S rDNA序列分析，发现它们构成了与红喉丹毒（Erysipelothrix rhusiopathae）和丝状霍尔德曼（Holdemania filiformis）相关的系统发育树上低G+C cramm阳性分支的一个新分支。提出了一个新属和一种。酵母在肉汤培养基中的生长较差，但在添加果糖、葡萄糖或麦芽糖的同时添加吐温80能促进酵母的生长。发酵葡萄糖和麦芽糖，水解精氨酸。醋酸盐、乳酸盐和微量琥珀酸盐是葡萄糖发酵的最终产物。该型菌株DNA中G+C含量为38 mol%。刺虫的类型菌株为DSM 13220(T)。
Five strains of anaerobic non-sporing Cram-positive bacilli isolated from advanced periodontitis (four strains) and a dentoalveolar abscess tone strain) that did not correspond to existing species were subjected to phenotypic and genetic characterization. Following 16S rDNA sequence analysis, they were found to constitute a novel branch of the low G+C Cram-positive division of the phylogenetic tree related to Erysipelothrix rhusiopathiae and Holdemania filiformis. A new genus Bulleidia, and the species Bulleidia extructa, are proposed. Growth of B. extructa in broth media was poor but was enhanced by the addition of fructose, glucose or maltose together with Tween 80. Glucose and maltose were fermented and arginine was hydrolysed. Acetate, lactate and trace amounts of succinate were the end products of glucose fermentation. The G+C content of the DNA of the type strain is 38 mol%. The type strain of Bulleidia extructa is DSM 13220(T).