Polymorphism of the pig 2,4-dienoyl CoA reductase 1 gene (DECR1) and its association with carcass and meat quality traits.
Polymorphism of the pig 2,4-dienoyl CoA reductase 1 gene (DECR1) and its association with carcass and meat quality traits.
复制标题
DOI：
10.2527/2005.833493x
复制
发表时间：
2005-03
影响因子：
3.3
通讯作者：
M. Amills;O. Vidal;L. Varona;A. Tomàs;M. Gil;A. Sánchez;J. Noguera
中科院分区：
文献类型：
--
作者：
M. Amills;O. Vidal;L. Varona;A. Tomàs;M. Gil;A. Sánchez;J. Noguera
我们鉴定了猪2,4-二烯基辅酶a还原酶1 （DECR1）基因的几乎完整编码序列，该基因编码一种参与多不饱和脂肪烯基辅酶a酯β -氧化的酶，并定位于位于4号染色体上的亚油酸QTL。对包含外显子2和10的937 bp片段的测序显示，外显子2 （C181—> G181）和外显子5 （C458—>G458）存在两个错义SNP。这两个SNP分别与DECR1蛋白61位和153位的Val (C) -> Leu (G)和Ser (C) -> Thr (G)保守AA替换相关。此外，对184头来自大白猪、Pietrain猪、伊比利亚猪、杜洛克猪和长白猪的代表性样本进行DECR1基因分型，结果表明这两个SNP（单倍型1:C181C458；单倍型2:G181G458）之间存在不平衡连锁。在一个高度选择的长白种人群体（n = 470）中，DECR1基因型与生长、胴体和肉质性状之间的关联分析显示，不同基因型在异柠檬酸脱氢酶活性（最高后密度[HPD]为90%）、胸最长肌pH （HPD为95%）、亮度（HPD为90 - 95%）和发红（HPD为95%）方面存在差异。由于这些关联在三个可用的基因型比较中并不一致，我们认为DECR1基因外显子2和5的多态性可能与影响异柠檬酸脱氢酶活性、肌肉颜色和pH值的真正因果突变存在连锁不平衡。
We characterized the nearly complete coding sequence of the pig 2,4-dienoyl CoA reductase 1 (DECR1) gene, which encodes an enzyme involved in the beta-oxidation of polyunsaturated fatty enoyl-CoA esters and maps on a linoleic QTL located on Chromosome 4. Sequencing of a 937-bp fragment encompassing exons 2 and 10 revealed the existence of two missense SNP at exon 2 (C181 --> G181) and exon 5 (C458 -->G458). These two SNP are associated with Val (C) --> Leu (G) and Ser (C) --> Thr (G) conservative AA replacements at positions 61 and 153 of the DECR1 protein, respectively. Moreover, DECR1 genotyping in a representative sample of 184 pigs from the Large White, Pietrain, Iberian, Duroc, and Landrace breeds demonstrated the existence of disequilibrium linkage between these two SNP (Haplotype 1: C181C458; Haplotype 2: G181G458). An association analysis between DECR1 genotype and growth, carcass, and meat quality traits in a highly selected Landrace population (n = 470) revealed differences among genotypes for isocitrate dehydrogenase activity (highest posterior density [HPD] of 90%), longissimus thoracis pH (HPD of 95%), lightness (HPD of 90 to 95%), and redness (HPD of 95%). Because these associations were not consistently found in the three available genotype comparisons, we believe that exon 2 and 5 polymorphisms at the DECR1 gene might be in linkage disequilibrium with the true causal mutation influencing isocitrate dehydrogenase activity and muscle color and pH.