AN ULTRASONOGRAPHIC METHOD FOR THE EVALUATION OF DEXMEDETOMIDINE ON MICTURITION IN INTACT RATS
AN ULTRASONOGRAPHIC METHOD FOR THE EVALUATION OF DEXMEDETOMIDINE ON MICTURITION IN INTACT RATS
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DOI：
10.1016/1056-8719(94)90090-6
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发表时间：
1994-12-01
影响因子：
1.9
通讯作者：
BENEDEK, G
中科院分区：
文献类型：
--
作者：
HORVATH, G;MORVAY, Z;BENEDEK, G
我们描述了一种简单、无创、无创伤、可重复的超声方法，以确定麻醉剂量（300 μ g/kg皮下注射）的α（2）-肾上腺素受体激动剂右美托咪定对完整大鼠排尿反射的影响。膀胱容积由椭球方程估计。为了验证该方法的可靠性，还进行了体外模型评估。估计体积和滴注体积之间的平均差异为27.3 μ L（-21.61，76.23）。高度显著的相关性（r = 0.98，p <0.001）表明超声检查与方程是一种可靠的工具。在对完整大鼠给予右美托咪定后，在30 +/- 4.8分钟时出现尿滴。排尿量阈值为2100 +/- 100 μ L。尽管几乎连续观察到尿液滴下，但在任何时间（10 - 100 min）获得的膀胱体积之间均未观察到显著差异。本研究表明，α 2-肾上腺素受体激动剂右美托咪定抑制了完整大鼠的排尿反射。所描述的方法，这是既无创和无痛，因此，可广泛用于量化在小动物的药理作用对膀胱。
We describe a simple, noninvasive, nontraumatic, and reproducible ultrasonographic method to determine the effect of the alpha(2)-adrenoceptor agonist dexmedetomidine in anesthetic dose (300 mu g/kg subcutaneously) on the micturition reflex in intact rats. The bladder volumes were estimated by an ellipsoid equation. To validate the reliability of the method, an in vitro model assessment also was performed. The mean difference between estimated and instilled volumes were 27.3 mu L (-21.61, 76.23). The highly significant correlation (r = 0.98, p < .001) indicates that the ultrasonography with the equation is a reliable tool.After the dexmedetomidine administration to intact rats, urine dribbling occurred at 30 +/- 4.8 min. The volume threshold for urination was 2100 +/- 100 mu L. Although dribbling of the urine was observed almost continuously, significant differences were not observed between bladder volumes obtained at any time (from 10 to 100 min).This study indicates that the alpha 2-adrenoceptor agonist dexmedetomidine inhibits the micturition reflex in intact rats. The method described, which is both noninvasive and nonpainful, may therefore be widely used to quantify in small animals pharmacological effects on the urinary bladder.