Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes.
Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes.
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DOI：
10.1084/jem.184.3.963
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发表时间：
1996-09-01
期刊：
影响因子：
--
通讯作者：
Moser B
中科院分区：
文献类型：
--
作者：
Loetscher M;Gerber B;Loetscher P;Jones SA;Piali L;Clark-Lewis I;Baggiolini M;Moser B
对CXC趋化因子IP 10和Mig具有选择性的人受体进行克隆和表征。该受体cDNA具有1104-bp的开放阅读框架，编码368个氨基酸的蛋白质，分子量为40，659道尔顿。该序列包括七个推定的G蛋白偶联受体特征性跨膜片段。它与两种IL-8受体共享40.9%和40.3%的相同氨基酸，与五种已知的CC趋化因子受体共享34.2%-36.9%的相同性。IP 10/Mig受体在IL-2激活的T淋巴细胞中高度表达，但在静息T淋巴细胞中检测不到。B淋巴细胞、单核细胞和粒细胞。它介导响应于IP 10和Mig的Ca 2+动员和趋化性，但不识别CXC-趋化因子IL-8、GRO α、NAP-2、GCP-2。ENA 78、PF 4、CC-趋化因子MCP-1、MCP-2、MCP-3、MCP-4、MIP-1 α、MIP-1 β。RANTES，1309，嗜酸性粒细胞趋化因子，nor嗜酸性粒细胞趋化因子。在活化的T淋巴细胞中的排他性表达是高度感兴趣的，因为迄今为止已经显示趋化因子的受体吸引淋巴细胞，例如，MCP-1、MCP- 2、MCP-3、MIP-1 α、MIP-1 β和RANTES也存在于单核细胞和粒细胞中。目前的观察表明，IP 10/Mig受体参与效应T细胞的选择性募集。
A human receptor that is selective for the CXC chemokines IP10 and Mig was cloned and characterized. The receptor cDNA has an open reading frame of 1104-bp encoding a protein of 368 amino acids with a molecular mass of 40,659 dalton. The sequence includes seven putative transmembrane segments characteristic of G-protein coupled receptors. It shares 40.9 and 40.3% identical amino acids with the two IL-8 receptors, and 34.2-36.9% identity with the five known CC chemokine receptors. The IP10/Mig receptor is highly expressed in IL-2-activated T lymphocytes, but is not detectable in resting T lymphocytes. B lymphocytes, monocytes and granulocytes. It mediates Ca2+ mobilization and chemotaxis in response to IP10 and Mig, but does not recognize the CXC-chemokines IL-8, GRO alpha, NAP-2, GCP-2. ENA78, PF4, the CC- chemokines MCP-1, MCP-2, MCP-3, MCP-4, MIP-1 alpha, MIP-1 beta. RANTES, 1309, eotaxin, nor lymphotactin. The exclusive expression in activated T-lymphocytes is of high interest since the receptors for chemokines which have been shown so far to attract lymphocytes, e.g., MCP-1, MCP- 2, MCP-3, MIP-1 alpha, MIP-1 beta, and RANTES, are also found in monocytes and granulocytes. The present observations suggest that the IP10/Mig receptor is involved in the selective recruitment of effector T cells.