Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells
Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells
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DOI：
10.1634/stemcells.2005-0648
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发表时间：
2006-08-01
期刊：
影响因子：
5.2
通讯作者：
Bouhassira, Eric E.
中科院分区：
文献类型：
--
作者：
Olivier, Emmanuel N.;Rybicki, Anne C.;Bouhassira, Eric E.
间充质干细胞 (MSC) 是多能祖细胞，存在于许多结缔组织中，包括脂肪、骨骼、软骨和肌肉。我们在此报告了一种无需使用任何饲养层即可将人胚胎干细胞 (hESC) 可重复分化为 MSC 的方法。通过此程序获得的细胞在形态上与骨髓 MSC 相似，是接触抑制的，可以在培养物中生长约 20 至 25 代，具有与骨髓 MSC 相似的免疫表型（CD34 和 CD45 阴性，CD13、CD44、CD71、CD73、CD105、CD166、人白细胞抗原 [HLA]-ABC 阳性，并且 阶段特异性胚胎抗原[SSEA]-4），可分化为骨细胞和脂肪细胞，可作为饲养细胞支持未分化hESC的生长。从 hESC 中产生 MSC 的能力应该有助于产生大量基因相同和可基因修饰的 MSC，这些 MSC 可用于研究 MSC 的生物学和治疗应用。
Mesenchymal stem cells (MSCs) are multipotent progenitors that can be found in many connective tissues, including fat, bone, cartilage, and muscle. We report here a method to reproducibly differentiate human embryonic stem cells (hESCs) into MSCs that does not require the use of any feeder layer. The cells obtained with this procedure are morphologically similar to bone marrow MSCs, are contact-inhibited, can be grown in culture for about 20 to 25 passages, have an immunophenotype similar to bone marrow MSCs (negative for CD34 and CD45 and positive for CD13, CD44, CD71, CD73, CD105, CD166, human leukocyte antigen [HLA]-ABC, and stage-specific embryonic antigen [SSEA]-4), can differentiate into osteocytes and adipocytes, and can be used as feeder cells to support the growth of undifferentiated hESCs. The ability to produce MSCs from hESCs should prove useful to produce large amounts of genetically identical and genetically modifiable MSCs that can be used to study the biology of MSCs and for therapeutic applications.