The role of a semi-automated NanoVelcro system in capturing circulating tumor cells and evaluating their prognostic value for gestational choriocarcinoma
The role of a semi-automated NanoVelcro system in capturing circulating tumor cells and evaluating their prognostic value for gestational choriocarcinoma
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半自动 NanoVelcro 系统在捕获循环肿瘤细胞并评估其对妊娠绒毛膜癌的预后价值中的作用
DOI：
10.1039/c8bm01130c
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发表时间：
2019
影响因子：
6.6
通讯作者：
Yi Z
中科院分区：
文献类型：
--
作者：
Minzhi Hou;Yongjiang Zheng;Zhiming Ding;Shanyang He;Manman Xu;Xinlin Chen;Hui Zhang;Chao Zeng;Cong Sun;Wenting Jiang;Han Wang;Hongwei Shen;Yang Zhang;Jing Liu;Shijun Sun;Neng Jiang;Yongmei Cui;Yu Sun;Yangshan Chen;Jessica Cao;Chunlin Wang;Mengzhen Li;Yi Z
探讨妊娠绒毛膜癌（GC）患者循环肿瘤细胞（CTC）的检出情况，并评价CTC计数对预后的预测价值。在这项多中心研究中，使用半自动NanoVelcro系统检测了180例GC患者的CTC存在，其中106例患者在一个化疗周期后进行了CTC重新评估。大约96%的GC患者在7.5 mL血液中含有≥2个ctc。远端转移患者（n = 95，范围0 ~ 104）每7.5 mL血液中ctc的数量远高于无远端转移患者（n = 85，范围0 ~ 6）。通过90例患者训练和90例患者验证队列，将ctc≥6的临界值定义为无进展生存期（PFS）和总生存期（OS）的预后阈值。≥6个ctc的存在与较差的PFS和OS显著相关（P < 0.001）。多因素分析显示，CTC数（≥6个CTC）是OS的最强预测因子（风险比[HR]， 15.8； 95%可信区间[CI]， 4.3-57.9; P < 0.001）。化疗一个周期后CTCs数量减少；单因素分析表明，第一个化疗周期后的CTC计数是OS的一个强有力的预测因子（HR, 36.1; 95% CI, 4.8-271.5; P < 0.001）。ctc是一种很有希望的GC预后因素。在这种疾病的背景下，一个周期化疗后的绝对CTC计数是化疗反应的一个强有力的预测指标。
To investigate whether circulating tumor cells (CTCs) are detectable in patients with gestational choriocarcinoma (GC) and evaluate the prognostic value of CTC enumeration. In this multicenter study, the presence of CTCs was examined in 180 GC patients using a semi-automated NanoVelcro system, among whom 106 patients underwent CTC re-evaluation after one cycle of chemotherapy. Approximately 96% of the GC patients contained ≥2 CTCs in 7.5 mL of blood. The number of CTCs per 7.5 mL of blood was much higher in patients with distant metastases (n = 95; range, 0 to 104) than in patients without distant metastases (n = 85; range, 0 to 6). Applying a 90-patient training and 90-patient validation cohort, a cutoff value of ≥6 CTCs was defined as the prognostic threshold for progression-free survival (PFS) and overall survival (OS). The presence of ≥6 CTCs was significantly associated with worse PFS and OS (both P < 0.001). A multivariate analysis showed that the CTC number (≥6 CTCs) was the strongest predictor of OS (hazard ratio [HR], 15.8; 95% confidence interval [CI], 4.3-57.9; P < 0.001). The number of CTCs decreased after one cycle of chemotherapy; univariate analyses demonstrated that the CTC count after the first chemotherapy cycle was a strong predictor of OS (HR, 36.1; 95% CI, 4.8-271.5; P < 0.001). CTCs are a promising prognostic factor for GC. The absolute CTC count after one cycle of chemotherapy in the context of this disease is a strong predictor of chemotherapy response.