EXPRESSION OF C-KIT GENE-PRODUCTS IN KNOWN CELLULAR TARGETS OF W-MUTATIONS IN NORMAL AND W-MUTANT MICE - EVIDENCE FOR AN IMPAIRED C-KIT KINASE IN MUTANT MICE
EXPRESSION OF C-KIT GENE-PRODUCTS IN KNOWN CELLULAR TARGETS OF W-MUTATIONS IN NORMAL AND W-MUTANT MICE - EVIDENCE FOR AN IMPAIRED C-KIT KINASE IN MUTANT MICE
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DOI：
10.1101/gad.3.6.816
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发表时间：
1989-06-01
影响因子：
10.5
通讯作者：
BESMER, P
中科院分区：
文献类型：
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作者：
NOCKA, K;MAJUMDER, S;BESMER, P
原癌基因c-kit是一种未知配体的跨膜酪氨酸蛋白激酶受体，最近被证明定位于小鼠的显性白色斑点位点（W）。W基因座的突变影响造血的各个方面，以及发育期间原始生殖细胞和成黑素细胞的增殖和/或迁移。在这里，我们表明，c-kit是在已知的胎儿和成人红细胞生成组织，肥大细胞和神经嵴衍生的黑色素细胞的W突变的影响组织中表达。我们证明，c-kit相关的酪氨酸特异性蛋白激酶在W/Wv肥大细胞功能受损，从而提供了一个分子基础，了解这些突变导致的发育缺陷。
The proto-oncogene c-kit, a transmembrane tyrosine protein kinase receptor for an unknown ligand, was shown recently to map to the dominant white spotting locus (W) of the mouse. Mutations at the W locus affect various aspects of hematopoiesis, as well as the proliferation and/or migration of primordial germ cells and melanoblasts during development. Here, we show that c-kit is expressed in tissues known to be affected by W mutations in fetal and adult erythropoietic tissues, mast cells, and neural-crest-derived melanocytes. We demonstrate that the c-kit associated tyrosine-specific protein kinase is functionally impaired in W/Wv mast dcells, thus providing a molecular basis for understanding the developmental defects that result from these mutations.