Genetic factors in lipoprotein metabolism. Analysis of a genetic cross between inbred mouse strains NZB/BINJ and SM/J using a complete linkage map approach.
Genetic factors in lipoprotein metabolism. Analysis of a genetic cross between inbred mouse strains NZB/BINJ and SM/J using a complete linkage map approach.
复制标题
脂蛋白代谢的遗传因素。
DOI：
10.1172/jci118230
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Lusis,AJ
中科院分区：
文献类型：
--
作者：
Purcell-Huynh,DA;Weinreb,A;Castellani,LW;Mehrabian,M;Doolittle,MH;Lusis,AJ
用血浆脂蛋白水平差异显著的两个亲本近交系NZB/BINJ和SM/J进行遗传杂交。测定了184名F2后代在正常饮食和致动脉粥样硬化饮食下的血脂和载脂蛋白水平。在除Y染色体以外的所有染色体上的126个座位上进行了遗传标记分型。统计分析显示脂蛋白水平与许多染色体上的标记显著连锁或暗示连锁。第1染色体标记与总胆固醇(LOD5.9)和高密度脂蛋白胆固醇(HDL8.1)水平连锁，第5染色体标记与总胆固醇(LOD6.7)和高密度脂蛋白胆固醇(HDL5.6)水平连锁，第7染色体标记与总甘油三酯(LOD5.1)和游离脂肪酸(LOD5.6)水平连锁。血浆apoAII水平与apoAII基因连锁(LOD19.6)，且与血浆高密度脂蛋白水平高度相关(r=0.63，P=0.0001)，表明apoAII表达影响高密度脂蛋白水平。分子研究表明，两个菌株apoAII多肽的结构差异可能是导致蛋白质清除率不同的原因。
A genetic cross was constructed from two parental inbred strains of mice, NZB/BINJ and SM/J, which differ markedly in their plasma lipoprotein levels. Plasma lipid and apolipoprotein values were measured in 184 F2 progeny on a normal chow diet and on an atherogenic diet. Genetic markers were typed at 126 loci spanning all chromosomes except the Y. Statistical analysis revealed significant linkage or suggestive linkage of lipoprotein levels with markers on a number of chromosomes. Chromosome 1 markers were linked to levels of total cholesterol (lod 5.9) and high density lipoprotein (HDL) cholesterol (lod 8.1), chromosome 5 markers were linked to levels of total cholesterol (lod 6.7) and HDL cholesterol (lod 5.6), and chromosome 7 markers were linked to levels of total plasma triglycerides (lod 5.1) and free fatty acids (lod 5.6). Plasma apoAII levels were linked to the apoAII gene (lod score 19.6) and were highly correlated with plasma HDL cholesterol levels (r = 0.63, P = 0.0001), indicating that apoAII expression influences HDL cholesterol levels. Molecular studies suggested that structural differences in the apoAII polypeptide of the two strains may contribute to differences in clearance of the protein.Images