REGULATION OF HEPATITIS-B VIRUS GENE-EXPRESSION BY ITS 2 ENHANCERS
REGULATION OF HEPATITIS-B VIRUS GENE-EXPRESSION BY ITS 2 ENHANCERS
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DOI：
10.1073/pnas.89.7.2708
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发表时间：
1992-04-01
影响因子：
11.1
通讯作者：
YEE, JK
中科院分区：
文献类型：
--
作者：
SU, H;YEE, JK
B型肝炎病毒（HBV）感染可引起急性和慢性肝炎，并与肝细胞癌的发生密切相关。 HBV感染的主要部位是肝脏，并且HBV在肝细胞中活跃地复制。 HBV基因组的两个区域先前已显示出转录增强子的特性。 在这项研究中，我们发现，两个HBV增强子中的任何一个都可以激活几个人肝癌细胞系中的所有三个主要HBV启动子，并且两个增强子的协同作用最终影响三个启动子的整体活性。 此外，我们的数据表明，HBV基因表达可能受到其增强子的差异调节。 HBV感染引起慢性肝脏炎症和肝细胞再生。 已经提出，在再生性增生期间突变的进行性积累可能导致肝细胞分化状态的改变。 因此，两个差异调节增强子的发展可能反映了HBV在肝细胞再生或肝癌发生过程中在分化程度较低的肝细胞中有效复制的策略。
Hepatitis B virus (HBV) infection causes acute and chronic hepatitis and is closely associated with the development of hepatocellular carcinoma. The principal site of HBV infection is liver, and HBV actively replicates in hepatocytes. Two regions of the HBV genome have been shown previously to display properties of a transcriptional enhancer. In this study, we show that either of the two HBV enhancers can activate all three major HBV promoters in several human hepatoma lines, and the cooperative action of the two enhancers ultimately affects overall activity of the three promoters. In addition, our data suggest that HBV gene expression may be differentially regulated by its enhancers. HBV infection causes chronic liver inflammation and hepatocyte regeneration. It has been proposed that progressive accumulation of mutations during the regenerative hyperplasia may lead to alterations in the differentiation state of hepatocytes. Thus, the development of two differentially regulated enhancers may reflect a strategy of HBV to replicate efficiently in less differentiated hepatocytes during hepatocyte regeneration or hepatocarcinogenesis.