GRANULOCYTE MACROPHAGE-COLONY-STIMULATING FACTOR STIMULATES BOTH ASSOCIATION AND ACTIVATION OF PHOSPHOINOSITIDE 3OH-KINASE AND SRC-RELATED TYROSINE KINASE(S) IN HUMAN MYELOID DERIVED CELLS
GRANULOCYTE MACROPHAGE-COLONY-STIMULATING FACTOR STIMULATES BOTH ASSOCIATION AND ACTIVATION OF PHOSPHOINOSITIDE 3OH-KINASE AND SRC-RELATED TYROSINE KINASE(S) IN HUMAN MYELOID DERIVED CELLS
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DOI：
10.1002/j.1460-2075.1993.tb05929.x
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发表时间：
1993-07-01
期刊：
影响因子：
11.4
通讯作者：
STEPHENS, LR
中科院分区：
文献类型：
--
作者：
COREY, S;EGUINOA, A;STEPHENS, LR
GM-CSF受体使用的信号通路目前尚不清楚。在此，我们表明，在人髓系衍生细胞中，GM-CSF可以刺激：（i）PtdIns（3，4，5）P3的积累;（ii）分别在抗lyn和抗c-yes抗体定向的免疫沉淀物中p53/p56 lyn和p62 c-yes定向的蛋白酪氨酸激酶活性的增加;（iii）在抗磷酸酪氨酸、抗p53/p56 lyn和抗p62 c-yes抗体导向的免疫沉淀物中磷酸肌醇3OH-激酶活性增加。这些结果表明，GM-CSF可以刺激蛋白酪氨酸激酶协调的信号传导复合物的形成，该复合物含有p53/p56 lyn、p62 c-yes和活化的PtdInsP 2定向的磷酸肌醇3OH-激酶，其可以驱动推定的第二信使PtdIns（3，4，5）P3的积累。
The signalling pathways used by the GM-CSF receptor are currently unknown. Here we show that in human myeloid derived cells GM-CSF can stimulate; (i) the accumulation of PtdIns(3,4,5)P3; (ii) increases in p53/p56lyn and p62c-yes directed protein tyrosine kinase activities in anti-lyn and anti-c-yes antibody directed immunoprecipitates, respectively and; (iii) increases in phosphoinositide 3OH-kinase activity in anti-phosphotyrosine, anti-p53/p56lyn and anti-p62c-yes antibody directed immunoprecipitates. These results suggest that GM-CSF can stimulate formation of protein tyrosine kinase co-ordinated signalling complexes, that contain p53/p56lyn, p62c-yes and an activated PtdInsP2 directed phosphoinositide 3OH-kinase, which can drive the accumulation of the putative second-messenger PtdIns(3,4,5)P3.