Nanosize and vitality:: TiO2 nanotube diameter directs cell fate
Nanosize and vitality:: TiO2 nanotube diameter directs cell fate
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DOI：
10.1021/nl070678d
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发表时间：
2007-06-01
期刊：
影响因子：
10.8
通讯作者：
Schmuki, Patrik
中科院分区：
文献类型：
--
作者：
Park, Jung;Bauer, Sebastian;Schmuki, Patrik
我们产生，在钛表面，自组装层的垂直取向的TiO 2纳米管与定义的直径在15和100 nm之间，并表明间充质干细胞的粘附，扩散，生长和分化是严重依赖于管直径。与光滑的TiO 2表面相比，小于30 nm的间距（最大值为15 nm）提供了加速整联蛋白聚集/焦点接触形成的有效长度尺度，并强烈增强了细胞活性。在管径大于50 nm的纳米管层上，细胞粘附和扩散严重受损，导致细胞活性显著降低和高度的程序性细胞死亡。因此，在TiO 2纳米管表面上，具有30-50 nm开口的横向间隔几何形状代表细胞命运的临界边界。
We generated, on titanium surfaces, self-assembled layers of vertically oriented TiO2 nanotubes with defined diameters between 15 and 100 nm and show that adhesion, spreading, growth, and differentiation of mesenchymal stem cells are critically dependent on the tube diameter. A spacing less than 30 nm with a maximum at 15 nm provided an effective length scale for accelerated integrin clustering/focal contact formation and strongly enhances cellular activities compared to smooth TiO2 surfaces. Cell adhesion and spreading were severely impaired on nanotube layers with a tube diameter larger than 50 nm, resulting in dramatically reduced cellular activity and a high extent of programmed cell death. Thus, on a TiO2 nanotube surface, a lateral spacing geometry with openings of 30-50 nm represents a critical borderline for cell fate.