Genotypes of JC virus in east, central and southwest Europe
Genotypes of JC virus in east, central and southwest Europe
复制标题
DOI：
10.1099/0022-1317-82-5-1221
复制
发表时间：
2001-05-01
影响因子：
3.8
通讯作者：
Stoner, GL
中科院分区：
文献类型：
--
作者：
Agostini, HT;Deckhut, A;Stoner, GL
JC病毒的独特基因型已在主要的大陆陆块中描述。对欧洲裔美国人和欧洲小队列的研究显示主要是1型。2型和7型在亚洲，3型和6型在非洲。这些基因型在序列上相差约1- 3%。每种基因型可具有彼此相差约0.5- 1%的若干亚型。基因型可以通过VP 1基因分型区域中核苷酸的独特模式来定义。这种基因分型方法已被证实的系统发育重建使用整个基因组的重排调控区除外。在这第一个大的欧洲研究中，我们报告了来自波兰，匈牙利，德国和西班牙的350个人的尿排泄ICV DNA。我们的研究对象包括匈牙利（罗姆人）、德国（辛提人）和西班牙（吉塔诺人）的吉普赛人，以及西班牙的巴斯克人。我们发现，虽然1型在欧洲占主导地位，1A型和1B型的比例可能会有所不同，从东欧到西南欧。与1型序列密切相关的4型（仅相似于1%的差异）在德国、波兰和西班牙是次要的基因型，但在巴斯克人中占多数。西班牙的吉塔诺人显示了一种变异的4型序列，称为“Rom-1”。有趣的是，无论是西班牙的吉塔诺人，还是德国或匈牙利的辛提人或罗姆人，都没有表现出2型或7型基因型，而如果他们的起源是亚洲人群，那么可能会出现这种基因型。
Distinctive genotypes of JC virus have been described for the major continental landmasses. Studies on European-Americans and small cohorts in Europe showed predominantly Type 1. Types 2 and 7 are found in Asia, and Types 3 and 6 in Africa. These genotypes differ in sequence by about 1-3 %. Each genotype may have several subtypes which differ from each other by about 0.5-1 %. The genotypes can be defined by a distinctive pattern of nucleotides in a typing region of the VP1 gene. This genotyping approach has been confirmed by phylogenetic reconstruction using the entire genome exclusive of the rearranging regulatory region. In this first large European study, we report on the urinary excretion of ICV DNA of 350 individuals from Poland, Hungary, Germany and Spain. We included Gypsy cohorts in Hungary (Roma), Germany (Sinti), and Spain (Gitano), as well as Basques in Spain. We show that while Type 1 predominates in Europe, the proportions of Type 1A and 1B may differ from East to Southwest Europe. Type 4, closely related to the Type 1 sequence (only similar to1% difference) was a minor genotype in Germany, Poland and Spain, but represented the majority in Basques. The Gitanos in Spain showed a variant Type 4 sequence termed 'Rom-1'. Interestingly, neither the Gitanos in Spain, nor Sinti or Roma in Germany or Hungary showed the Type 2 or Type 7 genotype that might be expected if their origins were in an Asian population.