A myelin protein is encoded by the homologue of a growth arrest-specific gene.
A myelin protein is encoded by the homologue of a growth arrest-specific gene.
复制标题
髓磷脂蛋白由生长停滞特异性基因的同源物编码。
DOI：
10.1073/pnas.88.16.7195
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发表时间：
1991
影响因子：
11.1
通讯作者：
Shooter,EM
中科院分区：
文献类型：
--
作者：
Welcher,AA;Suter,U;DeLeon,M;Snipes,GJ;Shooter,EM
坐骨神经损伤后细胞反应的显著特征是远端神经残端雪旺细胞的增殖和髓鞘特异性基因表达的下调。一旦轴突再生，雪旺细胞就会再次分化以改造髓鞘。我们已经分离到一个大鼠的cDNASR13，它在坐骨神经损伤后的早期阶段强烈下调。该基因编码一种糖蛋白，它与纯化的髓鞘蛋白有惊人的氨基酸相似性，并由髓鞘特异的抗血清沉淀。使用多肽特异性多克隆抗体的免疫组织化学实验将SR13蛋白定位于坐骨神经的髓鞘。计算机辅助序列分析发现，SR13与生长停滞特异性mRNA(Gas-3)有显著的同源性，该基因在静息状态下表达，但在增殖中的3T3小鼠成纤维细胞中不表达。在大鼠坐骨神经雪旺细胞增殖过程中，SR13的表达也同样下调。在两个不同的实验系统中，SR13和Gas-3mRNA与非增殖细胞的联系表明，这些分子在维持静止的细胞状态方面具有共同的作用。
Striking features of the cellular response to sciatic nerve injury are the proliferation of Schwann cells in the distal nerve stump and the downregulation of myelin-specific gene expression. Once the axons regrow, the Schwann cells differentiate again to reform the myelin sheaths. We have isolated a rat cDNA, SR13, which is strongly downregulated in the initial phase after sciatic nerve injury. This cDNA encodes a glycoprotein that shares striking amino acid similarity with a purified myelin protein and is specifically precipitated by a myelin-specific antiserum. Immunohistochemistry experiments using peptide-specific polyclonal antibodies localize the SR13 protein to the myelin sheath of the sciatic nerve. Computer-aided sequence analysis identified a pronounced homology of SR13 to a growth arrest-specific mRNA (Gas-3) that is expressed in resting but not in proliferating 3T3 mouse fibroblasts. SR13 is similarly downregulated during Schwann cell proliferation in the rat sciatic nerve. The association of the SR13 as well as the Gas-3 mRNA with nonproliferating cells in two different experimental systems suggests a common role for these molecules in maintaining the quiescent cell state.