Induction of Conjugation by Methyl Cellulose in Paramecium
Induction of Conjugation by Methyl Cellulose in Paramecium
复制标题
草履虫中甲基纤维素的结合诱导
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
N. Haga
中科院分区：
文献类型：
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作者：
A. Yanagi;N. Haga
建立了一种改进的草履虫自交偶联诱导方法。甲基纤维素在所有被测物种中都能简单有效地诱导自交偶联。甲基纤维素在尾状假体中诱导偶联，只发生在性成熟的交配反应细胞中。偶联产生有性后代，核过程的时间过程与互补型细胞之间的自然偶联基本相同。该方法可用于多种草履虫的遗传研究，包括尾状草履虫、四片草履虫、多微核草履虫和囊状草履虫。
ABSTRACT An improved method has been developed for the induction of selfing conjugation in Paramecium. Methyl cellulose induces selfing conjugation simply and efficiently in all species examined. Induction of conjugation by methyl cellulose was characterized in P. caudatum, where it occurred only in sexually mature, mating reactive cells. Conjugation produced sexual offspring and the time course of nuclear processes was substantially the same as that in natural conjugation between cells of complementary mating types. The method is useful for genetic studies in a wide variety of Paramecium species including P. caudatum, P. tetraurelia, P. multimicronucleatum and P. bursaria.
影响因子：
1.6
作者：
LYNN, DH;SOGIN, ML
通讯作者：
SOGIN, ML