Measurement of mRNA of trophoblast-specific genes in cellular and plasma components of maternal blood
Measurement of mRNA of trophoblast-specific genes in cellular and plasma components of maternal blood
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DOI：
10.1136/jmg.2005.040634
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发表时间：
2006-09-01
影响因子：
4
通讯作者：
Okai, T.
中科院分区：
文献类型：
--
作者：
Okazaki, S.;Sekizawa, A.;Okai, T.
背景：孕妇血浆中胎盘mRNA适合于定量分析，不受胎儿性别和遗传多态状态的影响。方法：采集155例孕妇外周血，比较母血细胞和血浆中人胎盘催乳素(HPL)和人绒毛膜促性腺激素β亚基(βhCG)的mRNA和蛋白水平。为了评估HPL mRNA表达的清除性，我们采集了9名剖宫产妇女在分娩前和分娩后的血样。结果：细胞成分中BHCG信使核糖核酸浓度与血浆中BHCG蛋白和BHCG信使核糖核酸浓度呈正相关(p=0.001)。血浆HPL蛋白浓度与细胞成分HPL mRNA浓度呈正相关(p<；0.05)。对于HPL和BHCG，细胞成分的mRNA浓度都高于血浆成分(HPL和BHCG分别高22.9倍和4.3倍)。细胞部分(平均半衰期为203.8~3465min)的半衰期明显长于血浆部分(平均半衰期为32.2min，范围为15~385min)(p=0.008)。结论：母血细胞成分中HPL和BHCG mRNA的浓度显著高于血浆成分。母血中的细胞信使核糖核酸有助于胎盘功能的非侵入性评估。
Background: Placental mRNA in maternal plasma is suitable for quantitative analysis regardless of fetal gender and genetic polymorphism status.Methods: We obtained 155 blood samples from pregnant women to compare human placental lactogen (hPL) and beta-subunit of human chorionic gonadotropin (beta hCG) mRNA and protein levels between the cellular and plasma components of maternal blood. To assess clearance of hPL mRNA expression, we obtained blood samples from nine women immediately before and after delivery by caesarean section. mRNA was extracted from the cellular and plasma components of all samples, and hPL and bhCG mRNA expression was analysed by reverse transcription-PCR assay.Results: The concentration of bhCG mRNA in the cellular component positively correlated with the plasma concentration of bhCG protein and bhCG mRNA (p = 0.001 for both). The concentration of hPL protein in the plasma correlated with the hPL mRNA concentration of the cellular component (p < 0.05). For both hPL and bhCG, the mRNA concentration of the cellular component was greater than that of the plasma component (22.9-fold higher for hPL and 4.3-fold higher for bhCG). The half life of hPL mRNA clearance was significantly longer for the cellular fraction (mean half life = 203.8 min, range 150 - 3465 min) than for the plasma fraction (mean half life = 32.2 min, range 15 - 385 min) (p = 0.008).Conclusion: The present findings indicate that the concentration of hPL and bhCG mRNA is significantly higher in the cellular component of maternal blood samples than in the plasma component. Cellular mRNA in maternal blood is useful for non-invasive evaluation of placental function.