GEM - AN INDUCED, IMMEDIATE-EARLY PROTEIN BELONGING TO THE RAS FAMILY
GEM - AN INDUCED, IMMEDIATE-EARLY PROTEIN BELONGING TO THE RAS FAMILY
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DOI：
10.1126/science.7912851
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发表时间：
1994-07-08
期刊：
影响因子：
56.9
通讯作者：
KELLY, K
中科院分区：
文献类型：
--
作者：
MAGUIRE, J;SANTORO, T;KELLY, K
从有丝分裂原诱导的人外周血T细胞中克隆了编码35kodalton鸟苷三磷酸(GTP)结合蛋白Gem的基因。GEM和Rad是II型糖尿病患者骨骼肌过表达基因的产物，构成了一个新的RAS相关GTP结合蛋白家族。该家族的独特结构特征包括G3GTP结合基序，广泛的氨基和羧基末端延伸超出RAS相关结构域，以及决定膜结合的基序。GEM在人外周血T细胞中瞬时表达，作为有丝分裂刺激的反应；该蛋白在酪氨酸残基上被磷酸化，并定位于质膜的胞浆表面。失控的Gem表达抑制了正常和转化的3T3细胞的增殖。这些结果表明，Gem是一种调节蛋白，可能参与了受体介导的质膜信号转导。
A gene encoding a 35-kilodalton guanosine triphosphate (GTP)-binding protein, Gem, was cloned from mitogen-induced human peripheral blood T cells. Gem and Rad, the product of a gene overexpressed in skeletal muscle in individuals with Type II diabetes, constitute a new family of Ras-related GTP-binding proteins. The distinct structural features of this family include the G3 GTP-binding motif, extensive amino- and carboxyl-terminal extensions beyond the Ras-related domain, and a motif that determines membrane association. Gem was transiently expressed in human peripheral blood T cells in response to mitogenic stimulation; the protein was phosphorylated on tyrosine residues and localized to the cytosolic face of the plasma membrane. Deregulated Gem expression prevented proliferation of normal and transformed 3T3 cells. These results suggest that Gem is a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane.