LAMININ AND FIBRONECTIN IN CELL-ADHESION - ENHANCED ADHESION OF CELLS FROM REGENERATING LIVER TO LAMININ
LAMININ AND FIBRONECTIN IN CELL-ADHESION - ENHANCED ADHESION OF CELLS FROM REGENERATING LIVER TO LAMININ
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DOI：
10.1073/pnas.78.4.2403
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
RUOSLAHTI, E
中科院分区：
文献类型：
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作者：
CARLSSON, R;ENGVALL, E;RUOSLAHTI, E
层粘连蛋白是从小鼠内皮细胞和大鼠卵黄囊癌细胞培养中分离得到的一种基底膜糖蛋白，它能促进小鼠再生肝细胞的附着。来自正常小鼠肝脏的细胞很容易附着在涂有纤维连接蛋白的培养皿上，但很难附着在涂有层粘连蛋白的表面。这两种蛋白有效地促进了细胞在再生过程中的附着。再生后，对层粘连蛋白的附着恢复到未接受肝部分切除的动物的低水平，但对纤维连接蛋白的附着仍然很高。用抗层粘连蛋白对正常肝脏切片进行免疫荧光染色，发现层粘连蛋白存在于胆管和血管壁内或邻近胆管和血管壁。肝部分切除诱导再生后，肝窦内层粘连蛋白增多。在四氯化碳中毒后，中央静脉周围的坏死区和坏死区的层粘连蛋白染色尤其明显。这种额外的层粘连蛋白表达是短暂的。伤后5~6天达高峰，随后逐渐消失。层粘连蛋白显然是一种粘附性蛋白质。再生肝脏中层粘连蛋白的增加以及肝细胞对层粘连蛋白的粘附性提示，层粘连蛋白在肝再生过程中维持适当的组织结构中起着重要作用。
Laminin, a basement membrane glycoprotein isolated from cultures of mouse endodermal cells and rat yolk sac carcinoma cell, promoted the attachment of liver cells obtained from regenerating mouse liver. Cells from normal mouse liver attached readily to dishes coated with fibronectin but attached poorly to surfaces coated with laminin. The 2 proteins efficiently promoted the attachment of cells from livers undergoing regeneration. Afer regeneration, the attachment to laminin returned to the low levels found in animals not subjected to partial hepatectomy, but attachment to fibronectin remained high. Immunofluorescent staining of sections of normal liver with antilaminin revealed the presence of laminin in or adjacent to the walls of the bile ducts and blood vessels. After induction of regeneration by partial hepatectomy, increased amounts of laminin appeared in the sinusoidal areas. After carbon tetrachloride poisoning, staining for laminin was especially pronounced in the necrotic and postnecrotic areas around the central veins. This additional expression of laminin was transient. It reached a maximum around 5-6 days after the injury and then gradually disappeared. Laminin is evidently an adhesive protein. The increase of laminin in regenerating liver and the adhesiveness of cells from such livers to laminin suggest a role for laminin in the maintenance of a proper tissue organization during liver regeneration.