THE HUMAN HEMATOPOIETIC PROGENITOR-CELL ANTIGEN (CD34) IN VASCULAR NEOPLASIA
THE HUMAN HEMATOPOIETIC PROGENITOR-CELL ANTIGEN (CD34) IN VASCULAR NEOPLASIA
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DOI：
10.1093/ajcp/96.1.25
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发表时间：
1991-07-01
影响因子：
3.5
通讯作者：
BATTIFORA, H
中科院分区：
文献类型：
--
作者：
TRAWEEK, ST;KANDALAFT, PL;BATTIFORA, H
人类造血祖细胞抗原CD34由早期正常造血祖细胞和许多急性白血病合成和表达。据报道，抗 CD34 抗体也会对组织切片中的血管进行染色，并且最近在血管内皮细胞中检测到了 CD34 mRNA。因此，作者研究了免疫组织化学 CD34 抗原检测在内皮细胞衍生肿瘤中的诊断效用，并将结果与冯维勒布兰德 (vW) 因子菌株进行了比较。还检查了多种上皮和间质肿瘤，以评估 CD34 对血管肿瘤的特异性。 7 例血管肉瘤（7 例中的 7 例）、5 例卡波西肉瘤（5 例中的 5 例）和 8 例上皮样血管内皮瘤（8 例中的 8 例）的 CD34 呈中度至强阳性。这种反应在冷冻切片、酒精固定的组织和福尔马林固定的标本中同样强烈。在许多情况下，恶性内皮细胞比邻近的良性内皮细胞染色更强烈。此外，在大多数情况下，CD34 阳性在数量和质量上都强于 vW 因子染色。两例血管外皮细胞瘤（两例中的两例）呈 CD34 阳性，但染色强度低于血管肉瘤、卡波西肉瘤或血管内皮瘤。 6 例血管瘤中有 5 例 CD34 染色也呈阳性；该组中的非反应性肿瘤是所研究的 28 种血管肿瘤中唯一对 CD34 没有反应性的肿瘤。相比之下，28 个血管肿瘤中有 9 个未对 vW 因子进行染色。还检查了三百五十七个非血管来源的肿瘤的 CD34 抗原表达。 1例肺鳞状细胞癌可见局灶性光染色；在所研究的一半上皮样肉瘤（16 例中的 8 例）和少数平滑肌肉瘤（22 例中的 3 例）中观察到中度至重度染色。这些发现表明CD34是血管源性肿瘤的敏感且相对特异的标记物。
The human hematopoietic progenitor cell antigen CD34 is synthesized and expressed by early normal hematopoietic progenitor cells and by many acute leukemias. Anti-CD34 antibodies also have been reported to stain blood vessels in tissue sections, and, more recently, CD34 mRNA has been detected in vascular endothelial cells. Therefore, the authors studied the diagnostic utility of immunohistochemical CD34 antigen detection in tumors of endothelial cell derivation and compared the results with strains for von Willebrand (vW) factor. A wide variety of epithelial and mesenchymal neoplasms also were examined to assess the specificity of CD34 for vascular neoplasia. Seven cases of angiosarcoma (seven of seven), five cases of Kaposi's sarcoma (five of five), and eight cases of epithelioid hemangioendothelioma (eight of eight) were moderately to strongly positive for CD34. This reactivity was equally intense in frozen sections, alcohol-fixed tissue, and formalin-fixed specimens. In many cases, the malignant endothenal cells stained more strongly than adjacent benign endothelium. Moreover, in most cases CD34 positivity was quantitatively and qualitatively stronger than staining for vW factor. Two cases of hemangiopericytoma (two of two) were CD34 positive but stained less intensely than the angiosarcomas, kaposi's sarcomas, or hemangioendotheliomas. Five of six cases of hemangioma also stained positively for CD34; the nonreactive tumor in this group was the only one among 28 vascular neoplasms studied that was not reactive for CD34. In comparison, 9 of the 28 vascular tumors did not stain for vW factor. Three hundred fifty-seven tumors of nonvascular derivation also were examined for CD34 antigen expression. Focal light staining was seen in one pulmonary squamous cell carcinoma; moderate to intense staining was observed in half of the epithelioid sarcomas studied (8 of 16) and in a minority of leiomyosarcomas (3 of 22). These findings indicate that CD34 is a sensitive and relatively specific marker for neoplasms of vascular origin.