The emerging role of nanotechnology in plant genetic engineering
The emerging role of nanotechnology in plant genetic engineering
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DOI：
10.1038/s44222-023-00037-5
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发表时间：
2023-02
期刊：
影响因子：
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通讯作者：
Henry J. Squire;Sophia Tomatz;Elizabeth Voke;Eduardo González-Grandío;M. Landry
中科院分区：
文献类型：
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作者：
Henry J. Squire;Sophia Tomatz;Elizabeth Voke;Eduardo González-Grandío;M. Landry
鉴于气候变化和人口增长压力，提高植物能力的基因工程至关重要。目前的操纵方法非常费力且依赖于物种，这限制了农业和分子农业的进步。因此，需要新的方法和工具来扩大可转化物种的范围并提高实现转化的通量。纳米技术彻底改变了微生物和动物系统的传输、传感和成像，但其在植物中的应用仍然很少。然而，用于植物遗传操纵的纳米介导传递的报道已经出现，包括直接种系编辑以及质体和线粒体基因组修饰。在这里，我们回顾了纳米技术在植物遗传操纵中的应用，包括用于传递遗传货物的纳米载体的开发以及纳米介导的植物再生的进展。特别关注理解合理设计纳米载体的结构-功能关系，以及这些发展如何催化植物生物技术应用的核酸和蛋白质递送的进展。
Genetic engineering to improve the capabilities of plants is essential given climate change and population growth pressures. Current manipulation methods are laborious and species dependent, which limits advances in agriculture and molecular farming. Therefore, new approaches and tools are needed to broaden the range of transformable species and increase the throughput at which transformation is achieved. Nanotechnology has revolutionized delivery, sensing and imaging in microbial and animal systems, but its application in plants remains scant. However, reports of nano-mediated delivery for the genetic manipulation of plants have emerged, including direct germline editing as well as plastid and mitochondrial genome modification. Here, we review the application of nanotechnology to plant genetic manipulation, including the development of nanocarriers for the delivery of genetic cargos and advances in nano-mediated plant regeneration. Particular focus is given to understanding structure–function relationships for the rational design of nanocarriers, and how these developments can catalyse progress in nucleic acid and protein delivery for plant biotechnology applications.