Dickkopf related genes are components of the positional value gradient in hydra
Dickkopf related genes are components of the positional value gradient in hydra
复制标题
DOI：
10.1016/j.ydbio.2006.04.003
复制
发表时间：
2006-08-01
影响因子：
2.7
通讯作者：
Bosch, Thomas C. G.
中科院分区：
文献类型：
--
作者：
Augustin, Rene;Franke, Andre;Bosch, Thomas C. G.
水螅是了解再生和轴形成等基本发育生物学过程的经典模式生物。在这里，我们发现，有一些相似性的Dickkopf家族的蛋白质，HyDkk 1/2/4-A和HyDkk 1/2/4-C的成员，基因共表达在腺细胞和调节的位置值梯度。虽然HyDkk 1/2/4-A在整个胃区域表达，但HyDkk 1/2/4-C具有分级表达模式，在腱区下方具有高水平的转录物，并且在出芽区不表达。阻断GSK-3 β的活性导致胃组织中HyDkk 1/2/4-C表达的急剧下调。实验减少HyDkk 1/2/4-C表达细胞的数量导致hypostome中HyWnt表达结构域的扩增。因此，类似于脊椎动物中的Dickkopf蛋白，水螅中HyDkk 1/2/4-C的功能之一可能是拮抗Wnt信号传导。(c)2006年爱思唯尔公司All rights reserved.
Hydra is a classical model organism to understand fundamental developmental biological processes such as regeneration and axis formation. Here, we show that two genes which share some similarity with members of the Dickkopf family of proteins, HyDkk1/2/4-A and HyDkk1/2/4-C, are co-expressed in gland cells and regulated by the positional value gradient. While HyDkk1/2/4-A is expressed throughout the gastric region, HyDkk1/2/4-C has a graded expression pattern with a high level of transcripts just below the tentacle zone and absence of expression in the budding zone. Blocking the activity of GSK-3 beta caused a drastic downregulation of HyDkk1/2/4-C expression in the gastric tissue. Experimental reduction of the number of HyDkk1/2/4-C-expressing cells resulted in expansion of the HyWnt expression domain in the hypostome. Thus, similar to Dickkopf proteins in vertebrates, one of the functions of HyDkk1/2/4-C in hydra may be to antagonize Wnt signalling. (c) 2006 Elsevier Inc. All rights reserved.