Influence of microparticle formulation on immunogenicity of SYI, a synthetic peptide derived from Streptococcus mutans GbpB.
Influence of microparticle formulation on immunogenicity of SYI, a synthetic peptide derived from Streptococcus mutans GbpB.
复制标题
微粒制剂对 SYI（一种源自变形链球菌 GbpB 的合成肽）免疫原性的影响。
DOI：
10.1111/j.1399-302x.2004.00191.x
复制
发表时间：
2005
影响因子：
--
通讯作者：
Smith,DJ
中科院分区：
文献类型：
--
作者：
Peacock,ZS;Barnes,LA;King,WF;Trantolo,DJ;Wise,DL;Taubman,MA;Smith,DJ
Subcutaneous immunization with SYI, a peptide construct based onStreptococcus mutansglucan binding protein B (GbpB) residues 113–132, significantly reduces experimental dental caries. Since mucosal immunization may be preferred for human vaccine applications, the present objective was to determine what formulation of SYI combined with polylactide‐coglycolide microparticles could give rise to significant levels of salivary IgA antibody reactive with the native GbpB protein. A comparison of the SYI construct, loaded into or mixed with polylactide‐coglycolide revealed the SYI‐loaded microparticles to induce significant and sustainable levels of salivary and nasal wash IgA antibody to the peptide and the native protein. SYI mixed with unloaded microparticles was less effective in mucosal antibody response induction. These studies indicate that mucosal immunization with the SYI construct can induce salivary IgA antibody to a pathogenesis‐associated component ofS. mutansif delivered within polylactide‐coglycolide microparticles, suggesting that this approach could successfully induce protective salivary immunity to dental caries caused byS. mutans.
