Specific interaction between Lex and Lex determinants. A possible basis for cell recognition in preimplantation embryos and in embryonal carcinoma cells.
Specific interaction between Lex and Lex determinants. A possible basis for cell recognition in preimplantation embryos and in embryonal carcinoma cells.
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发表时间：
1989-06
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通讯作者：
Ivan Eggens;B. Fenderson;T. Toyokuni;B. Dean;M. Stroud;Sen-itiroh Hakomori
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作者：
Ivan Eggens;B. Fenderson;T. Toyokuni;B. Dean;M. Stroud;Sen-itiroh Hakomori
Lex 决定簇 (Gal beta 1----4[Fuc alpha 1----3]GlcNAc-beta 1----R) 已被认为在介导桑葚胚阶段小鼠胚胎的压缩中发挥作用(Fenderson, B., Zehavi, U., and Hakomori, S. (1984) J. Exp. Med. 160, 1591-1596)。在这里，我们提供的证据表明 Lex 在 F9 胚胎癌细胞粘附中的作用以及基于碳水化合物-碳水化合物相互作用的 Lex 识别机制。 F9 细胞的同型聚集被乳-N-岩藻五糖 III 抑制，并且 F9 细胞显示出与 Lex 脂质体的优先相互作用。以下观察结果表明，能够识别 F9 细胞上的 Lex 本身的结构是 Lex：（i）溶解在辛基葡萄糖苷中的细胞表面标记成分，亲和结合在 Lex-辛基-Sepharose 柱上，含有与抗 Lex 抗体反应的糖蛋白。 (ii) 含有 Lex 的脂质体显示出与涂在塑料表面上的 Lex 糖脂（但与其他糖脂）显着的相互作用。 (iii)发现含有Lex糖脂的脂质体会自聚集，而含有paragloboside (nLc4)或唾液酸paragloboside (IV3NeuAcnLc4)的脂质体则不会。 (iv)与Lex脂质体一起温育的3H标记的乳-N-岩藻五醇III（但不是I或II）通过半透膜从下Boyden室到上Boyden室的扩散性被抑制。在所有这些实验(i-iv)中，仅在Ca2+和Mg2+存在下清楚地观察到Lex与Lex（或Lex与乳-N-岩藻五糖III）的相互作用，并且通过Mn2+的存在而增强。这些相互作用被 EDTA 抑制。结果表明，碳水化合物-碳水化合物相互作用可能在 F9 细胞聚集和胚胎发育过程中控制细胞识别方面发挥重要作用。
The Lex determinant (Gal beta 1----4[Fuc alpha 1----3]GlcNAc-beta 1----R) has been implicated as having a role in mediating compaction of the mouse embryo at the morula stage (Fenderson, B., Zehavi, U., and Hakomori, S. (1984) J. Exp. Med. 160, 1591-1596). Here, we present evidence suggesting a role for Lex in F9 embryonal carcinoma cell adhesion and a mechanism for Lex recognition based on carbohydrate-carbohydrate interaction. Homotypic aggregation of F9 cells was inhibited by lacto-N-fucopentaose III, and F9 cells showed a preferential interaction with Lex liposomes. The following observations suggest that the structure capable of recognizing Lex per se on F9 cells is Lex: (i) Cell surface-labeled components solubilized in octylglucoside, affinity-bound on an Lex-octyl-Sepharose column, contained glycoproteins reactive with anti-Lex antibody. (ii) Liposomes containing Lex showed significant interaction with Lex glycolipid, but not other glycolipids, coated on a plastic surface. (iii) Liposomes containing Lex glycolipid were found to self-aggregate, whereas liposomes containing paragloboside (nLc4) or sialylparagloboside (IV3NeuAcnLc4) did not. (iv) The diffusibility of 3H-labeled lacto-N-fucopentaitol III (but not I or II), incubated with Lex liposome, from the lower to the upper Boyden chamber through a semipermeable membrane was inhibited. In all these experiments (i-iv), the interaction of Lex to Lex (or Lex to lacto-N-fucopentaose III) was clearly observed only in the presence of Ca2+ and Mg2+ and was enhanced by the presence of Mn2+. These interactions were inhibited by EDTA. The results suggest the novel hypothesis that carbohydrate-carbohydrate interactions may play an important role in controlling cell recognition during F9 cell aggregation and during embryonic development.