Comparative analysis of the transcriptional patterns of pathogenic and nonpathogenic porcine circoviruses
Comparative analysis of the transcriptional patterns of pathogenic and nonpathogenic porcine circoviruses
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DOI：
10.1016/s0042-6822(03)00096-5
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发表时间：
2003-05-25
期刊：
影响因子：
3.7
通讯作者：
Cheung, AK
中科院分区：
文献类型：
--
作者：
Cheung, AK
对猪圆环病毒1型(PCV1)在PK15细胞中合成的RNA进行了鉴定。共检测到12个RNA。它们包括病毒衣壳蛋白RNA(CR)，由8个Rep相关RNA(命名为Rep、Rep‘、Rep3a、Rep3b、Rep3c-1、Rep3c-2、Rep3c-3和Rep3c-4)组成的4簇，以及3个NS相关RNA(命名为NS462、NS642和NS0)。Rep相关RNA簇的成员都共享共同的5‘和3’核苷酸序列，并且他们与NS相关RNA共享共同的3‘核苷酸序列。Rep能够编码全长复制相关蛋白，似乎是通过交替剪接产生其他七个Rep相关RNA的主要转录本。NS462、NS642和NS0似乎是从存在于ORF1中的三个不同启动子转录而来，独立于Rep启动子。根据序列比对分析，非致病性PCV1和致病性猪圆环病毒2型(PCV2)(含有9个RNA：Rep、Rep‘、Rep3a、Rep3b、Rep3c、NS515、NS672和NS0)都利用位于基因组上相似的遗传元件来表达病毒基因。PCV1和PCV2的Rep、Rep‘、Rep3a、Rep3b和NS0在其各自的系统中被视为等价实体。然而，在Rep3c和NS相关的RNA之间观察到了数量和质量上的差异(剪接连接变异)。这项工作为研究PCV1和PCV2的生物学特性(和差异)提供了一般的框架和遗传学基础。(C)2003年埃尔塞维尔科学公司(美国)。版权所有。
The RNAs of porcine circovirus type 1 (PCV1) synthesized in PK15 cells were characterized. A total of 12 RNAs were detected. They include the viral capsid protein RNA (CR), 4 cluster of eight Rep-associated RNAs (designated Rep, Rep', Rep3a, Rep3b, Rep3c-1, Rep3c-2, Rep3c-3, and Rep3c-4), and three NS-associated RNAs (designated NS462, NS642, and NS0). Members of the Rep-associated RNA cluster all share common 5'- and 3'-nucleotide sequences and they share common 3'-nucleotide sequence with the NS-associated RNAs. Rep, capable of coding for the full-length replication-associated protein, appears to be the primary transcript that gives rise to the other seven Rep-associated RNAs by alternate splicing. NS462, NS642, and NS0 appear to have been transcribed from three different promoters present inside ORF1, independent from the Rep promoter. Based on sequence alignment analysis, both the nonpathogenic PCV1 and the pathogenic porcine circovirus type 2 (PCV2) (with nine RNAs: Rep, Rep', Rep3a, Rep3b, Rep3c, NS515, NS672, and NS0) utilize comparable genetic elements similarly located along the genome for viral gene expression. The Rep, Rep', Rep3a, Rep3b, and NS0 of PCV1 and PCV2 are considered equivalent entities in their respective systems. However, quantitative and qualitative differences (splice junction variation) were observed among the Rep3c- and NS-associated RNAs. This work provides a general framework and genetic basis to investigate the biologic properties (and differences) of PCV1 and PCV2. (C) 2003 Elsevier Science (USA). All rights reserved.