Molecular characterization of ONAC300, a novel NAC gene specifically expressed at early stages in various developing tissues of rice
Molecular characterization of ONAC300, a novel NAC gene specifically expressed at early stages in various developing tissues of rice
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DOI：
10.1007/s00438-004-1097-2
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发表时间：
2005-02-01
影响因子：
3.1
通讯作者：
Kadowaki, K
中科院分区：
文献类型：
--
作者：
Kusano, H;Asano, T;Kadowaki, K
植物特异性基因家族的成员被称为NAC家族（nam - ataf - cu -related），参与包括调控植物发育在内的多种功能。然而，目前还没有关于单子叶植物NAC家族任何成员的详细分子特征的报道。在这里，我们报道了ONAC300的这种特征，ONAC300是一个新的nac家族基因，利用克隆自水稻微解剖韧皮部细胞的cDNA鉴定。预测的ONAC300蛋白序列属于NAM亚群，该亚群还包含拟南芥中的CUC1和CUC2蛋白、金鱼龙中的CUP蛋白、南瓜中的CmNACP蛋白和矮牵牛中的NAM蛋白。原位杂交技术在发育中的茎尖分生组织（SAM）及其幼叶中检测到高水平的ONAC300 mRNA。使用ONAC300:: GUS报告基因发现，ONAC300启动子主要表达在发育中的叶和根维管组织中。花药花丝、花轴和心皮花柱也有这种结构。RT-PCR分析进一步揭示了ONAC300转录本在叶片、根和茎中的表达水平高于穗。观察到的ONAC300的表达模式与之前报道的dicot NAC基因有很大的不同。因此，ONAC300是NAC家族的一个新成员，在水稻的茎、根、花以及维管组织的成熟韧皮部的早期发育阶段就表达。
Members of the plant-specific gene family referred to as the NAC family (for NAM-ATAF-CUC-related) are involved in various functions including the regulation of plant development. However, no detailed molecular characterization of any member of the NAC family has yet been reported from monocots. Here, we report such a characterization of ONAC300, a novel NAC-family gene identified using a cDNA cloned from microdissected phloem cells of rice. The predicted ONAC300 protein sequence falls into the NAM subgroup, which also contains the proteins CUC1 and CUC2 from Arabidopsis, CUP from snapdragon, CmNACP from pumpkin and NAM from petunia. High levels of ONAC300 mRNA were detected by in situ hybridization in developing shoot apical meristem (SAM) and in the associated young leaves. The use of an ONAC300:: GUS reporter gene revealed that the ONAC300 promoter was expressed predominantly in developing vascular tissues of the leaves and roots. The construct was also expressed in anther filaments, rachis and carpel styles. RT-PCR analysis further revealed that the levels of ONAC300 transcripts were higher in leaves, roots and culms than in panicles. The observed expression pattern of ONAC300 is quite different from those of the dicot NAC genes previously reported. Thus, ONAC300 is a novel member of the NAC family which is expressed at very early developmental stages in the shoot, root and flower, as well as in the mature phloem of vascular tissues in rice.