Molecular evidence for nitrite-dependent anaerobic methane-oxidising bacteria in the Jiaojiang Estuary of the East Sea (China)
Molecular evidence for nitrite-dependent anaerobic methane-oxidising bacteria in the Jiaojiang Estuary of the East Sea (China)
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DOI：
10.1007/s00253-014-5556-3
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发表时间：
2014-02
影响因子：
5
通讯作者：
Lidong Shen;Zhu Qun;L. Shuai;Du Ping;Zeng Jiang-ning;Dong-qing Cheng;Xiangyang Xu;Zheng Ping-Zheng-Pin
中科院分区：
文献类型：
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作者：
Lidong Shen;Zhu Qun;L. Shuai;Du Ping;Zeng Jiang-ning;Dong-qing Cheng;Xiangyang Xu;Zheng Ping-Zheng-Pin
亚硝酸盐依赖的厌氧甲烷氧化（N-DAMO）是近年来发现的一个连接全球碳氮循环的过程。据报道，这一过程是由“Methylomirabilis oxyfera”介导的。到目前为止，M。在有限数量的淡水生境中检测到了类似oxyfera的细菌，但目前尚不清楚这些细菌是否存在于河口生境中。本研究对云南省的M.对东海椒江口沉积物中的类尖孢菌进行了研究。16 SrRNA和pmoA基因均证实了M. oxyfera-like细菌在检查河口。回收的16 S rRNA基因序列与M. pmoA基因与M. oxyfera的pmoA基因同源性为85.1- 95.4%。oxyfera。定量PCR进一步证实了M.在该河口中发现了一种类oxyfera-like细菌，其丰度变化范围为5.80 ± 0.28 × 104 ~ 8.35 ± 0.52 × 107 copies g（干重）−1。相关分析表明，沉积物有机质含量是影响M.在所调查的环境因子中，本研究首次证明了M.结果表明，该细菌的分布与河口环境条件有一定的相关性。
Nitrite-dependent anaerobic methane oxidation (N-DAMO) is a recently discovered process linking the global carbon and nitrogen cycles. This process was reported to be mediated by “Candidatus Methylomirabilis oxyfera”. To date,M. oxyfera-like bacteria have been detected in a limited number of freshwater habitats, but whether these bacteria occur in estuarine habitats is currently unknown. In this study, the distribution, diversity and abundance ofM. oxyfera-like bacteria were studied in the sediment of the Jiaojiang Estuary of the East Sea (China). Both the 16S ribosomal RNA (rRNA) andpmoAgenes confirmed the occurrence ofM. oxyfera-like bacteria in the examined estuary. The recovered 16S rRNA gene sequences showed 91.5–97.2 % identity to the 16S rRNA gene ofM. oxyfera, and the recoveredpmoAgene sequences showed 85.1–95.4 % identity to thepmoAgene ofM. oxyfera. Quantitative PCR further confirmed the occurrence ofM. oxyfera-like bacteria in this estuary, with the abundance varying from 5.80 ± 0.28 × 104to 8.35 ± 0.52 × 107copies g (dry weight)−1. Correlation analysis indicated that the sediment organic content was the most important factor affecting the distribution ofM. oxyfera-like bacterial communities in the examined sediments among the environmental factors investigated. This study demonstrated for the first time the existence ofM. oxyfera-like bacteria in an estuarine environment and showed the correlations between the distribution of these bacteria and the estuarine environmental conditions.