Synthesis and application of N-hydroxysuccinimidyl rhodamine B ester as an amine-reactive fluorescent probe
Synthesis and application of N-hydroxysuccinimidyl rhodamine B ester as an amine-reactive fluorescent probe
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DOI：
10.1016/j.dyepig.2005.12.008
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发表时间：
2007-01-01
影响因子：
4.5
通讯作者：
Huang, Deyin
中科院分区：
文献类型：
--
作者：
Meng, Qinghua;Yu, Meijuan;Huang, Deyin
以罗丹明B（RB）为原料，通过一步缩合反应合成了2位含N-羟基琥珀酰亚胺酯的荧光探针（RB-S）。研究了荧光探针与甘氨酸的反应性，并通过LC-MS分析鉴定了产物。过量的RB-S被水解成相应的羧酸（RB）。研究了荧光探针（RB-S）、标记衍生物（RB-Gly）和水解产物（RB）的紫外-维斯光谱和荧光光谱。荧光光谱和吸收光谱呈现为镜像。研究了甘氨酸衍生物（RB-Gly）在甲醇/水溶液中的溶剂效应。(c)2006爱思唯尔有限公司版权所有。
A fluorescent probe (RB-S) containing the N-hydroxysuccinimidyl ester in 2 position was prepared from rhodamine B (RB) by one-step condensation reaction. The reactivity of the fluorescent probe with glycine was studied and the products were identified by LC-MS analysis. The excessive equivalent of the RB-S was hydrolyzed to the corresponding carboxylic acids (RB). UV/vis and fluorescence spectra of the fluorescent probe (RB-S), the labeled derivative (RB-Gly) and the hydrolysate (RB) were also studied. Fluorescence and absorption spectra appear as mirror images. The solvent effect on the spectra of the glycine derivative (RB-Gly) was investigated in methanol/water solution. (c) 2006 Elsevier Ltd. All rights reserved.