What Does the Future Hold for Top Down Mass Spectrometry?
What Does the Future Hold for Top Down Mass Spectrometry?
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DOI：
10.1016/j.jasms.2009.10.014
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发表时间：
2010-02-01
影响因子：
3.2
通讯作者：
Garcia, Benjamin A.
中科院分区：
文献类型：
--
作者：
Garcia, Benjamin A.
质谱（MS）研究已经彻底改变了现代生物学和生物医学领域。大多数质谱实验的核心是使用自下而上的质谱方法，其中蛋白质在MS询问之前首先被蛋白水解成较小的片段。然而，电子捕获解离和最近的电子转移解离的出现使得自上而下（完整蛋白质的分析）或中间向下（大多肽的分析）质谱法在发育、生长和总体使用方面都有很大的增加。然而，对于高通量大规模蛋白质组学研究，自下而上质谱法很容易主导该领域。随着自上而下的质谱方法和技术的不断发展，它是否真的能够与自下而上的质谱法在整个蛋白质组分析中竞争？这里讨论的是目前的方法，应用，问题，和未来的高通量自顶向下质谱。（J Am Soc Mass Spectron2010，21，193-202）（C）2010由Elsevier Inc.美国质谱学会American Society for Mass Spectrometry
Mass spectrometry (MS) research has revolutionized modem biological and biomedical fields. At the heart of the majority of mass spectrometry experiments is the use of Bottom Up mass spectrometry methods where proteins are first proteolyzed into smaller fragments before MS interrogation. The advent of electron capture dissociation and, more recently, electron-transfer dissociation, however, has allowed Top Down (analysis of intact proteins) or middle down (analysis of large polypeptides) mass spectrometry to both experience large increases in development, growth, and overall usage. Nevertheless, for high-throughput large-scale proteomic studies, Bottom Up mass spectrometry has easily dominated the field. As Top Down mass spectrometry methodology and technology continue to develop, will it genuinely be able to compete with, Bottom Up mass spectrometry for whole proteome analysis? Discussed here are the current approaches, applications, issues, and future view of high-throughput Top Down mass spectrometry. (J Am Soc Mass Spectrorn 2010, 21, 193-202) (C) 2010 Published by Elsevier Inc. on behalf of American Society for Mass Spectrometry