Molecular architecture of the DNA-binding region and its relationship to classification of basic helix-loop-helix proteins
Molecular architecture of the DNA-binding region and its relationship to classification of basic helix-loop-helix proteins
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DOI：
10.1093/molbev/msl143
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发表时间：
2007-01-01
影响因子：
10.7
通讯作者：
Zhao, Jieping
中科院分区：
文献类型：
--
作者：
Atchley, William R.;Zhao, Jieping
多元统计分析用于探索基本螺旋-环-螺旋（bHLH）蛋白的dna结合和二聚化区域的分子结构。使用一组5个多变量“指数”将字母顺序氨基酸数据转换为具有生物学意义的定量值。这些多变量指标总结了大量氨基酸理化属性的变化，反映了极性-接近性-疏水性、二级结构倾向、分子大小、密码子组成和静电电荷的变化。利用这些指数评分数据，判别分析描述了生理化学变异的多维方面，并阐明了bHLH蛋白主流进化分类的结构基础。当同时考虑两个结合二聚化结构域的少量氨基酸时，可以准确地区分5个已知的dna结合基团。相关地点通常具有良好的结构和功能特征。
Multivariate statistical analyses are used to explore the molecular architecture of the DNA-binding and dimerization regions of basic helix-loop-helix (bHLH) proteins. Alphabetic amino acid data are transformed to biologically meaningful quantitative values using a set of 5 multivariate "indices." These multivariate indices summarize variation in a large suite of amino acid physiochemical attributes and reflect variability in polarity-accessibility-hydrophobicity, propensity for secondary structure, molecular size, codon composition, and electrostatic charge. Using these index score data, discriminant analyses describe the multidimensional aspects of physiochemical variation and clarify the structural basis of the prevailing evolutionary classification of bHLH proteins. A small number of amino acids from both the binding dimerization domains, when considered simultaneously, accurately distinguish the 5 known DNA-binding groups. The relevant sites often have well-documented structural and functional characteristics.