Paraquat-stimulated binding of dopa to liver and lung microsomal protein.
Paraquat-stimulated binding of dopa to liver and lung microsomal protein.
复制标题
百草枯刺激多巴与肝和肺微粒体蛋白的结合。
DOI：
10.3109/00498258009033802
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
D. Davies
中科院分区：
文献类型：
--
作者：
M. McManus;D. Davies
1. 3h -多巴被肺和肝微粒体转化为一种活性中间体，与肺和肝微粒体蛋白共价结合。2. 超氧化物歧化酶使3h -多巴与肺和肝微粒体结合的放射性降低到水煮微粒体的水平。3. 百草枯（5mm）使3h -多巴与肺微粒体结合的放射性增加133%，而与肝微粒体结合的放射性增加224%。然而，超氧化物歧化酶使其与肺微粒体和肝微粒体的结合分别降低38%和50%。4. 苯甲酸酯和1,4-重氮双环[2,2,2]辛烷在百草枯存在和不存在的情况下对3h -多巴的放射性结合没有影响。5. 谷胱甘肽和抗坏血酸在没有和存在百草枯的情况下使结合降低到低于煮沸微粒体的水平。6. 在百草枯存在的情况下，可溶性部分与微粒体蛋白的生理比例降低了结合，达到对照水平。
1. 3H-Dopa is converted by lung and liver microsomes to a reactive intermediate which binds covalently to lung and liver microsomal protein. 2. Binding of radioactivity from 3H-dopa to lung and liver microsomes was decreased by superoxide dismutase to the level observed with boiled microsomes. 3. Paraquat (5 mM) caused a 133% increase in binding of radioactivity from 3H-dopa with lung microsomes compared with 224% increase with liver microsomes. However, superoxide dismutase decreased the binding by 38% and 50% with lung and liver microsomes respectively. 4. Benzoate and 1,4-diazobicyclo[2,2,2]octane in the absence and presence of paraquat had no effect on the binding of radioactivity from 3H-dopa. 5. Glutathione and ascorbic acid in the absence and presence of paraquat decreased the binding to below the level obtained with boiled microsomes. 6. The soluble fraction when present in the physiological ratio to microsomal protein decreased the binding, in the presence of paraquat, to the level of the control.