EFFECTS OF PULSATILE FLOW ON CULTURED VASCULAR ENDOTHELIAL-CELL MORPHOLOGY
EFFECTS OF PULSATILE FLOW ON CULTURED VASCULAR ENDOTHELIAL-CELL MORPHOLOGY
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DOI：
10.1115/1.2891226
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发表时间：
1991-05-01
影响因子：
1.7
通讯作者：
NEREM, RM
中科院分区：
文献类型：
--
作者：
HELMLINGER, G;GEIGER, RV;NEREM, RM
内皮细胞（EC）出现，以适应其形态和功能，在体内血液动力学环境中，他们居住。体外实验表明，类似的改变发生在培养EC暴露于层流稳态流动诱导的剪切应力。然而，体内EC暴露于脉动流环境;因此，在本研究中，使用40 +/- 20达因/cm 2（I型）的1-Hz非反向正弦剪切应力，研究了脉动流对细胞形状和取向以及对融合的牛主动脉内皮细胞（BAEC）单层中肌动蛋白微丝定位的影响，1 Hz反向正弦剪切应力为20 +/- 40和10 +/- 15达因/cm 2（II型），1 Hz振荡剪切应力为0 +/- 20和0 +/- 40达因/cm 2（III型）。结果表明，在I型不可逆流动中，细胞形状变化不太迅速，但细胞呈现出比长期稳定流动控制更细长的形状。对于低振幅II型逆转流，BAEC的形状变化不太迅速，并且总是比其稳定的对照组更短;然而，对于高振幅逆转，BAEC没有保持附着超过24小时。对于III型振荡流，BAEC细胞的形状保持多边形的静态培养，并没有表现出肌动蛋白应力纤维，如发生在所有其他流量。这些结果表明，EC可以区分不同类型的脉动流环境。此外，这些实验表明工程化细胞培养环境的重要性，以便包括脉动流在血管内皮细胞生物学的调查，无论这些研究是设计来研究血管生物学和内皮细胞在疾病过程中的作用，或导致的混合，内皮细胞prescriptive血管移植物的发展。
Endothelial cells (EC) appear to adapt their morphology and function to the in vivo hemodynamic environment in which they reside. In vitro experiments indicate that similar alterations occur for cultured EC exposed to a laminar steady-state flow-induced shear stress. However, in vivo EC are exposed to a pulsatile flow environment; thus, in this investigation, the influence of pulsatile flow on cell shape and orientation and on actin microfilament localization in confluent bovine aortic endothelial cell (BAEC) monolayers was studied using a 1-Hz nonreversing sinusoidal shear stress of 40 +/- 20 dynes/cm2 (type I), 1-Hz reversing sinusoidal shear stresses of 20 +/- 40 and 10 +/- 15 dynes/cm2 (type II), and 1-Hz oscillatory shear stresses of 0 +/- 20 and 0 +/- 40 dynes/cm2 (type III). The results show that in a type I nonreversing flow, cell shape changed less rapidly, but cells took on a more elongated shape than their steady flow controls long-term. For low-amplitude type II reversing flow, BAECs changed less rapidly in shape and were always less elongated than their steady controls; however, for high amplitude reversal, BAECs did not stay attached for more than 24 hours. For type III oscillatory flows, BAEC cell shape remained polygonal as in static culture and did not exhibit actin stress fibers, such as occurred in all other flows. These results demonstrate that EC can discriminate between different types of pulsatile flow environments. Furthermore, these experiments indicate the importance of engineering the cell culture environment so as to include pulsatile flow in investigations of vascular endothelial cell biology, whether these studies are designed to study vascular biology and the role of the endothelial cell in disease processes, or are ones leading to the development of hybrid, endothelial cell-preseeded vascular grafts.