Alpha-fetoprotein producing cells act as cancer progenitor cells in human cholangiocarcinoma
Alpha-fetoprotein producing cells act as cancer progenitor cells in human cholangiocarcinoma
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DOI：
10.1016/j.canlet.2010.01.019
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发表时间：
2010-08-01
期刊：
影响因子：
9.7
通讯作者：
Uemoto, Shinji
中科院分区：
文献类型：
--
作者：
Ishii, Takamichi;Yasuchika, Kentaro;Uemoto, Shinji
我们的目的是证明胆管癌中产生甲胎蛋白（AFP）的细胞具有癌症干细胞（CSC）样特性。 AFP增强子/启动子驱动的EGFP基因被转染到人胆管癌细胞系中。一种细胞系 RBE 同时表达 AFP 和 EGFP。克隆分析显示，一种 EGFP 阳性细胞同时产生 EGFP 阳性和 EGFP 阴性细胞部分。然而，一个 EGFP 阴性细胞从未产生 EGFP 阳性细胞。 EGFP阳性细胞具有更大的致瘤潜力。仅 EGFP 阳性细胞表达 Notch1。在临床肝内胆管癌中观察到AFP和Notch1的表达。产生 AFP 的细胞被认为是 CSC。 Notch 通路可能在维持 CSC 特性方面发挥重要作用。 (C) 2010 Elsevier Ireland Ltd. 保留所有权利。
We aimed to demonstrate that alpha-fetoprotein (AFP)-producing cells in cholangiocarcinomas possessed cancer stem cell (CSC)-like properties. AFP enhancer/promoter-driven EGFP gene was transfected into human cholangiocarcinoma cell lines. One cell line, RBE, expressed both AFP and EGFP. Clonal analyses revealed that one EGFP-positive cell generated both EGFP-positive and EGFP-negative cell fractions. However, one EGFP-negative cell never produced EGFP-positive cells. The EGFP-positive cells had a greater tumorigenic potential. Only the EGFP-positive cells expressed Notch1. AFP and Notch1 expression was observed in clinical intrahepatic cholangiocarcinomas. The AFP-producing cells were suggested to be CSCs. The Notch pathway might play an important role in maintaining the CSC characteristics. (C) 2010 Elsevier Ireland Ltd. All rights reserved.