Recent advances in expanding the coverage of the lipidome.
Recent advances in expanding the coverage of the lipidome.
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DOI：
10.1016/j.copbio.2016.11.008
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发表时间：
2017-02
影响因子：
7.7
通讯作者：
Kamphorst JJ
中科院分区：
文献类型：
--
作者：
Tumanov S;Kamphorst JJ
脂质组的极端多样性构成了重大的分析挑战。存在多种基于质谱的方法用于分析脂质。结合提取和分离程序最大限度地覆盖脂质体。脂质组包含大量具有不同物理化学性质的分子。脂质是细胞的结构成分，作为能量来源，并作为信号介质发挥作用。脂质代谢的改变涉及多种疾病的发生和进展，包括代谢综合征和癌症。正因为如此，近年来对脂质组学（通过质谱法对脂质组进行全面表征）的兴趣有所增强。然而，由于其巨大的结构多样性，获得样品中所有脂质的真正完整概述仍然非常具有挑战性。在这里，我们提供了用于研究各种脂质类的分析方法的集合的概述，强调样品制备和液相色谱-质谱（LC-MS）的创新。此外，我们还提供了增加脂质体覆盖范围的实用建议。
The extreme diversity of the lipidome poses a significant analytical challenge. A variety of mass spectrometry-based approaches exist for the analysis of lipids. Combining extraction and separation procedures maximizes coverage of the lipidome. The lipidome comprises a large array of molecules with diverse physicochemical properties. Lipids are structural components of cells, act as a source of energy, and function as signaling mediators. Alterations in lipid metabolism are involved in the onset and progression of a variety of diseases, including metabolic syndrome and cancer. Because of this, interest in lipidomics, the comprehensive characterization of the lipidome by mass spectrometry, has intensified in recent years. However, obtaining a truly complete overview of all lipids in a sample has remained very challenging due to their enormous structural diversity. Here, we provide an overview of the collection of analytical approaches used to study various lipid classes, emphasizing innovations in sample preparation and liquid chromatography–mass spectrometry (LC–MS). Additionally, we provide practical suggestions for increasing the coverage of the lipidome.