Receptor tyrosine kinase Tie-1 overexpression in endothelial cells upregulates adhesion molecules
Receptor tyrosine kinase Tie-1 overexpression in endothelial cells upregulates adhesion molecules
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DOI：
10.1016/j.bbrc.2008.04.091
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发表时间：
2008-07-04
影响因子：
3.1
通讯作者：
Sukhatme, Vikas P.
中科院分区：
文献类型：
--
作者：
Chan, Barden;Yuan, Hai-Tao;Sukhatme, Vikas P.
Tie-1是一种内皮特异性细胞表面蛋白，其生物学特性尚不清楚。利用体外过表达系统，我们检测了内皮细胞中的Tie-1活性是否会引发促炎反应。我们发现，当Tie-1在体外内皮细胞中过表达时，酪氨酸磷酸化。我们还发现Tie-1部分通过p38依赖机制上调VCAM-1、e-选择素和ICAM-1。有趣的是，Tie-1对人主动脉内皮细胞中VCAM-1和e-选择素的上调明显高于脐静脉内皮细胞。此外，Tie-1的表达也增强了单核细胞对内皮细胞的附着。综上所述，我们的数据表明Tie-1具有促炎特性，可能在动脉粥样硬化等内皮炎性疾病中发挥作用。(C) 2008爱思唯尔公司版权所有。
Tie-1 is an endothelial specific cell surface protein whose biology remains poorly understood. Using an overexpression system in vitro, we examined whether Tie-1 activity in endothelial cells in vitro would elicit a proinflammatory response. We found that when overexpressed in endothelial cells in vitro, Tie-1 is tyrosine-phosphorylated. We also showed that Tie-1 upregulates VCAM-1, E-selectin, and ICAM-1, partly through a p38-dependent mechanism. Interestingly, upregulation of VCAM-1 and E-selectin by Tie-1 is significantly higher in human aortic endothelial cells than in human umbilical vein endothelial cells. Additionally, attachment of cells of monocytic lineage to endothelial cells is also enhanced by Tie-1 expression. Collectively, our data show that Tie-1 has a proinflammatory property and may play a role in the endothelial inflammatory diseases such as atherosclerosis. (C) 2008 Elsevier Inc. All rights reserved.