Shaping efects of rice, wheat, maize, and soybean seedlings on their rhizosphere microbial community
Shaping efects of rice, wheat, maize, and soybean seedlings on their rhizosphere microbial community
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水稻、小麦、玉米和大豆幼苗对其根际微生物群落的塑造作用
DOI：
10.1007/s11356-022-24835-3
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发表时间：
2022
影响因子：
5.8
通讯作者：
Qian Haifeng
中科院分区：
文献类型：
--
作者：
Zhang Fan;Xu Nuohan;Zhang Zhenyan;Zhang Qi;Yang Yaohui;Yu Zhitao;Sun Liwei;Lu Tao;Qian Haifeng
根际微生物群在植物生长过程中起着至关重要的作用，是植物与土壤环境进行资源交换的重要界面。作物不同生育期，特别是苗期，对根际微生物群落有很强的塑造作用，群落重建对植物生长有正反馈作用。本研究利用16S rRNA基因和内转录间隔区(ITS)高通量测序技术，分析了水稻、大豆、玉米和小麦四种作物在连续栽培(苗期三次重复)过程中根际细菌和真菌群落的变化。我们发现，在不同的培养时间后，特定微生物的相对丰度有所下降，如鞘氨醇单胞菌、假单胞菌、红假单胞菌和弧菌，这些已被报道为植物生长有益细菌。潜在植物病原菌Myrothecium和Ascochyta的相对丰度随着连续培养次数的增加而增加。共生网络分析表明，玉米下的细菌和真菌群落比水稻、大豆和小麦下的要稳定得多。本研究探讨了作物幼苗根际细菌和真菌的特征，表明土壤植物区系的特征可能决定了植物的生长状况。进一步的研究将集中在利用关键微生物来控制特定作物的生长和产量。
The rhizosphere microbiome plays critical roles in plant growth and is an important interface for resource exchange between plants and the soil environment. Crops at various growing stages, especially the seedling stage, have strong shaping effects on the rhizosphere microbial community, and such community reconstruction will positively feed back to the plant growth. In the present study, we analyzed the variations of bacterial and fungal communities in the rhizosphere of four crop species: rice, soybean, maize, and wheat during successive cultivations (three repeats for the seedling stages) using 16S rRNA gene and internal transcribed spacer (ITS) high-throughput sequencing. We found that the relative abundances of specific microorganisms decreased after different cultivation times, e.g.,Sphingomonas,Pseudomonas,Rhodanobacter, andCaulobacter, which have been reported as plant-growth beneficial bacteria. The relative abundances of potential plant pathogenic fungiMyrotheciumandAscochytaincreased with the successive cultivation times. The co-occurrence network analysis showed that the bacterial and fungal communities under maize were much more stable than those under rice, soybean, and wheat. The present study explored the characteristics of bacteria and fungi in crop seedling rhizosphere and indicated that the characteristics of indigenous soil flora might determine the plant growth status. Further study will focus on the use of the critical microorganisms to control the growth and yield of specific crops.