Effect of cadmium-induced oxidative stress on antioxidative enzymes in mitochondria and cytoplasm of CRL-1439 rat liver cells.
Effect of cadmium-induced oxidative stress on antioxidative enzymes in mitochondria and cytoplasm of CRL-1439 rat liver cells.
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DOI：
10.3892/ijmm.18.3.477
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发表时间：
2006-09
影响因子：
5.4
通讯作者：
L. Latinwo;V. Badisa;C. Ikediobi;Caroline O Odewumi;Ayuk-Takem T Lambert;R. Badisa
中科院分区：
文献类型：
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作者：
L. Latinwo;V. Badisa;C. Ikediobi;Caroline O Odewumi;Ayuk-Takem T Lambert;R. Badisa
镉通过职业和环境暴露影响人类健康。本文报道了不同浓度(0-150微米)的氯化镉在37℃作用24小时对CRL-1439细胞线粒体和细胞质抗氧化酶的影响。肝细胞暴露于50微米的氯化镉对线粒体过氧化氢酶和谷胱甘肽还原酶(GR)活性的增强作用大于对细胞质酶的作用。尽管线粒体硒依赖的谷胱甘肽过氧化物酶(Se-GPx)的酶活性低于细胞质酶，但线粒体硒非依赖性谷胱甘肽过氧化物酶(Non-Se-GPx)的活性比未处理的细胞质酶略有增加。与对照组相比，加入100微米氯化镉后，过氧化氢酶在线粒体中的比活性保持在细胞质酶的基础上。GR在细胞质中的水平高于线粒体。然而，线粒体中的Se-GPx和非Se-GPx的活性与细胞质中的相应酶相比略有下降。由于毒性作用，细胞暴露在150微米的氯化镉中，与100微米的氯化镉处理的样品相比，所有抗氧化酶的活性都降低了。当暴露于不同浓度的镉时，每种抗氧化酶都表现出自己的激活或抑制模式，线粒体中观察到更多的氧化应激。
Cadmium affects human health through occupational and environmental exposure. In this report, we present the response of mitochondrial and cytoplasmic antioxidant enzymes of CRL-1439 cells exposed to different concentrations (0-150 microM) of CdCl2 for 24 h at 37 degrees C. Exposure of liver cells to 50 microM CdCl2 increased mitochondrial catalase and glutathione reductase (GR) activities more than the cytoplasmic enzymes. Although the mitochondrial selenium-dependent glutathione peroxidase (Se-GPx) showed less enzymatic activity than the cytoplasmic enzyme, the mitochondrial selenium-independent glutathione peroxidase (non-Se-GPx) showed a slight increase in activity over its cytoplasmic counterpart compared to untreated controls. With 100 microM CdCl2, catalase maintained an increase in specific activity in mitochondria over the cytoplasmic enzyme compared to the controls. The level of GR was higher in the cytoplasm than in the mitochondria. However, the activity of Se-GPx and non-Se-GPx decreased slightly in the mitochondria compared to their cytoplasmic counterparts. Exposure of cells to 150 microM CdCl2 decreased all antioxidant enzyme activities compared to the 100 microM CdCl2-treated samples due to toxic effect. Each antioxidant enzyme exhibited its own pattern of activation or inhibition upon exposure to different concentrations of cadmium, with more oxidative stress observed in the mitochondria.