Icosahedral 60-meric porous structure of designed supramolecular protein nanoparticle TIP60
Icosahedral 60-meric porous structure of designed supramolecular protein nanoparticle TIP60
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设计的超分子蛋白质纳米颗粒TIP60的二十面体60聚体多孔结构
DOI：
10.1039/d1cc03114g
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发表时间：
2021
影响因子：
4.9
通讯作者：
Ryoichi Arai
中科院分区：
文献类型：
--
作者：
Junya Obata;Norifumi Kawakami;Akihisa Tsutsumi;Erika Nasu;Kenji Miyamoto;Masahide Kikkawa;Ryoichi Arai
超分子蛋白质纳米颗粒和纳米笼具有广泛的应用潜力。最近，我们开发了一种均匀的超分子蛋白质纳米颗粒，TIP60，由五聚体 Sm 样蛋白和二聚体 MyoX-coil 结构域的融合蛋白对称自组装而成。在此，我们报告了使用单粒子冷冻电子显微镜解析的 TIP60 的二十面体 60 聚体结构。有趣的是，该结构在表面显示出 20 个正三角形的孔。 TIP60 及其突变体在其外表面和内表面上有许多可修饰位点。 TIP60 架构将有助于开发未来应用的生物医学和生化纳米颗粒/纳米笼。
Supramolecular protein nanoparticles and nanocages have potential in a broad range of applications. Recently, we developed a uniform supramolecular protein nanoparticle, TIP60, symmmetrically self-assembled from fusion proteins of a pentameric Sm-like protein and a dimeric MyoX-coil domain. Herein, we report the icosahedral 60-meric structure of TIP60 solved using single-particle cryo-electron microscopy. Interestingly, the structure revealed 20 regular-triangle-like pores on the surface. TIP60 and its mutants have many modifiable sites on their exterior and interior surfaces. The TIP60 architecture will be useful in the development of biomedical and biochemical nanoparticles/nanocages for future applications.