Influence of cholesterol on liposome fluidity by EPR - Relationship with percutaneous absorption
Influence of cholesterol on liposome fluidity by EPR - Relationship with percutaneous absorption
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DOI：
10.1016/s0168-3659(00)00240-6
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发表时间：
2000-07-31
影响因子：
10.8
通讯作者：
Parra, JL
中科院分区：
文献类型：
--
作者：
Coderch, L;Fonollosa, J;Parra, JL
考察了脂质体组成对双层膜流动性的影响及其对透皮吸收的影响。制备了由饱和或不饱和磷脂（H-PC或PC）与不同量的胆固醇形成的脂质体，并通过剥离法跟踪了它们对角质层的渗透行为。利用电子顺磁共振（EPR）方法研究了囊泡疏水结构域的有序性和动力学。磷脂组成和胆固醇的量对包封在脂质体中的探针的渗透行为产生相当大的影响。这种行为是密切相关的，这些脂质体的流动性特征的EPR研究，因此，渗透机制的囊泡到皮肤中，脂质的掺入到皮肤脂质和流动性行为的基础上，建议。(C)2000 Elsevier Science BN。All rights reserved.
The influence of liposome composition on bilayer fluidity and its effect on the percutaneous absorption into the skin were investigated. Liposomes formed with saturated or unsaturated phospholipids (H-PC or PC) with varying amounts of cholesterol were prepared and their penetration behaviour into the stratum corneum was followed up by means of the stripping method. The order and dynamics of the hydrophobic domain of the vesicles were studied using electron paramagnetic resonance (EPR) methodology. Phospholipid composition and the amount of cholesterol exert a considerable influence on the penetration behaviour of the probe encapsulated in the liposomes. This behaviour is closely related to the fluidity characteristics of these liposomes studied by EPR, Therefore, a penetration mechanism of the vesicles into the skin, based on the incorporation of lipids into the skin lipids and on fluidity behaviour, is suggested. (C) 2000 Elsevier Science BN. All rights reserved.