Voltage-dependent inactivation of volume-regulated Cl- current in human T84 colonic and B-cell myeloma cell lines.
Voltage-dependent inactivation of volume-regulated Cl- current in human T84 colonic and B-cell myeloma cell lines.
复制标题
人 T84 结肠和 B 细胞骨髓瘤细胞系中容量调节 Cl 电流的电压依赖性失活。
DOI：
10.1007/bf00410203
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Garber,SS
中科院分区：
文献类型：
--
作者：
Levitan,I;Garber,SS
The voltage-dependent inactivation of a volume-regulated Cl−current (VRChlC) in an epithelial (T84), and a B cell myeloma (RPMI-8226) cell lines were compared. Both cell types exhibited similar time and voltage-dependent current inactivation when recorded using standard whole cell patch technique, with a NaCl external solution that was ∼90 mOsm hypotonic to the Cs-Glutamate internal solution. The time course of voltage-dependent current inactivation was fit by the sum of a slow, dominant voltage-dependent and a faster voltage-independent components. Recovery from inactivation was approximated by a single exponential process and was dependent on the recovery voltage. The major difference was that the voltage-dependent current inactivation in myeloma cells was shifted to a ∼30mV more depolarized value than that in T84 cells although the effective charge movement of inactivation was similar. These results indicate that the macroscopic volume-regulated Cl−current in these two cell lines is functionally similar.
