Genome-wide linkage mapping of QTL for physiological traits in a Chinese wheat population using the 90K SNP array
Genome-wide linkage mapping of QTL for physiological traits in a Chinese wheat population using the 90K SNP array
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DOI：
10.1007/s10681-016-1682-6
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发表时间：
2016-06-01
期刊：
影响因子：
1.9
通讯作者：
He, Zhonghu
中科院分区：
文献类型：
--
作者：
Gao, Fengmei;Liu, Jindong;He, Zhonghu
许多生理性状与小麦的谷物产量和产量相关性状有关。本研究的目的是确定生理性状的数量性状基因座（QTL），包括地被、植被指数的标准化差异和冠层温度降低，以及用于标记辅助育种的紧密连锁的分子标记。使用高密度 Illumina iSelect 90K 单核苷酸多态性 (SNP) 测定对来自 Zhou 8425B/Chinese Spring 杂交的 246 个 F-8 重组自交系 (RIL) 进行基因分型。 2012-2013年和2013-2014年种植季节在河南省郑州和周口在灌溉条件下进行了田间试验。生理性状方差分析显示 RIL 之间以及 RIL x 环境相互作用存在显着差异 (P < 0.01)。晚春地被植物 (GC-S)、晚春植被指数归一化差异 (NDVI-S) 和冬前地被植物 (GC-W) 的广义广义遗传力分别为 0.66、0.52 和 0.40。 GC-S、NDVI-S 和花后 10 天冠层温度降低 (CTD-10) 与 GY 呈正相关。利用5636个多态性SNP标记的高密度连锁图和复合区间作图，在12条染色体上鉴定出GC、NDVI和CTD的24个QTL，解释了3.4-14.6%的表型变异。在三种环境中检测到五个稳定的QTL，即。 QGC-W.caas-7AL、QNDVI-S.caas-7AL、QGC-S.caas-3AS、QCTD-A.caas-5BS 和 QCTD-10.caas-5BS。此外，在染色体1AL、2AL、2BL、3AS、3B(2)、4BS、5B、7AS和7AL上观察到10个QTL簇。稳定的QTL和QTL簇与SNP标记连锁，与最近的SNP的遗传距离范围为0至2.0 cM；这些可用于标记辅助选择，以改善小麦育种中的产量相关性状。
Many physiological traits are associated with grain yield and yield-related traits in wheat. The aim of the present study was to identify quantitative trait loci (QTL) for physiological traits, including ground cover, normalized difference in vegetation index and canopy temperature depression, and closely linked molecular markers for marker-assisted breeding. Two hundred and forty-six F-8 recombinant inbred lines (RILs) come from the Zhou 8425B/Chinese Spring cross were genotyped using the high-density Illumina iSelect 90K single nucleotide polymorphism (SNP) assay. Field trials were conducted under irrigated conditions at Zhengzhou and Zhoukou in Henan province, during the 2012-2013 and 2013-2014 cropping seasons. Analysis of variance of physiological traits showed significant differences (P < 0.01) among RILs, and RILs x environment interactions. Ground cover at late spring (GC-S), normalized difference in vegetation index at late spring (NDVI-S), and ground cover pre-winter (GC-W), had moderate broad-sense heritabilities of 0.66, 0.52 and 0.40, respectively. GC-S, NDVI-S and canopy temperature depression at 10 days post anthesis (CTD-10) were positively correlated with GY. Using a high-density linkage map of 5636 polymorphic SNP markers and composite interval mapping, 24 QTL for GC, NDVI and CTD were identified on 12 chromosomes, explaining 3.4-14.6 % of the phenotypic variance. Five stable QTL were detected across three environments, viz. QGC-W.caas-7AL, QNDVI-S.caas-7AL, QGC-S.caas-3AS, QCTD-A.caas-5BS and QCTD-10.caas-5BS. In addition, 10 QTL clusters were observed on chromosomes 1AL, 2AL, 2BL, 3AS, 3B (2), 4BS, 5B, 7AS, and 7AL. The stable QTL and QTL clusters were linked to SNP markers, with genetic distances to the closest SNPs ranging from 0 to 2.0 cM; these could be used for marker-assisted selection to improve yield-related traits in wheat breeding.